[Effects and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum]

To examine the impact and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum. The experimental technique was used. Ten eight-month-old male Sprague-Dawley (SD) rats have been taken to arrange regular rat serum (hereinafter known as regular serum), 30 eight-month-old male SD rats have been taken to arrange burn rat serum (hereinafter known as burn serum) 6 hours after full-thickness burn, and the third passage of epidermal cells collected from 10 one-day-old SD rats have been used for the experiments.

The cells have been divided into regular serum group handled with regular serum and burn serum group handled with burn serum. Cell counting package eight technique was used to detect cell survival fee after 1, 2, 4, 6, and eight h of tradition, respectively, to display the following intervention time of burn serum. The cells have been divided into burn serum management group handled solely with burn serum and 50, 100, 150, 200, 250 μmol/L sodium hydrosulfide teams handled with burn serum+sodium hydrosulfide at corresponding last molarity.

After 30 min of tradition following the burn serum intervention, the cell survival fee was measured as above to display the following intervention focus of sodium hydrosulfide. The cells have been divided into burn serum management group handled with burn serum solely and sodium hydrosulfide solely group, glibenclamide solely group, and sodium hydrosulfide+glibenclamide group handled with burn serum+corresponding reagents. After 5, 10, 15 min of tradition following the burn serum intervention, the cell survival fee was measured as above to display the following intervention time of glibenclamide. The cells have been divided into burn serum management group handled with burn serum and sodium hydrosulfide solely group, glibenclamide solely group, and sodium hydrosulfide+glibenclamide group handled with burn serum+corresponding reagents.

After finishing corresponding tradition time of every reagent, the mitochondria have been extracted to detect cytochrome c oxidase (CCO) exercise utilizing a spectrophotometer, and the protein expression stage of adenosine triphosphate (ATP)-sensitive potassium channel was detected by Western blotting. Except for the quantity of samples for ATP-sensitive potassium channel protein detection, which was 3, the quantity of samples for the opposite indicators was 10. Data have been statistically analyzed with evaluation of variance for factorial design, one-way evaluation of variance, least important distinction (LSD)-t take a look at, LSD take a look at, and Bonferroni correction.

Compared with that of regular serum group, the survival fee of cells was considerably decreased in burn serum group after solely 4 and 6 h of tradition (t=4.02, 6.42, P<0.05). There have been statistically important variations among the many time factors inside regular serum group and burn serum group in survival fee of cells (F=19.74, 4.48, P<0.05 or P<0.01). Culture of Four h was chosen as the following intervention time of burn serum. After 30 min of tradition following the burn serum intervention, in contrast with that of burn serum management group, solely the 150, 200, 250 μmol/L sodium hydrosulfide teams had a considerably larger survival fee of cells (P<0.05), thus 150 μmol/L was chosen as the following intervention focus of sodium hydrosulfide.

Compared with that of burn serum management group, the survival fee of cells decreased considerably in glibenclamide solely group after 5 and 15 min of tradition following the burn serum intervention (P<0.05) and elevated considerably in glibenclamide solely group after 10 min of tradition following the burn serum intervention and sodium hydrosulfide solely group at every time level (P<0.05 or P<0.01). The survival fee of cells in hydrosulfide+glibenclamide group was considerably decrease than that of sodium hydrosulfide solely group at every time level (P<0.05). There have been statistically important variations among the many time factors inside glibenclamide solely group in survival fee of cells (F=11.81, P<0.01).

Culture of 5 min was chosen as the following intervention time of glibenclamide. After 35 min of tradition following the burn serum intervention, in contrast with (1.62±0.08) nmol·min(-1)·mg(-1) and 0.68±0.06 in burn serum management group, the CCO exercise of cells and the protein expression stage of ATP-sensitive potassium channel have been considerably elevated in sodium hydrosulfide solely.

group ((1.99±0.09) nmol·min(-1)·mg(-1) and 0.93±0.01, P<0.01) and considerably decreased in glibenclamide solely group ((1.44±0.09) nmol·min(-1)·mg(-1) and 0.60±0.01, P<0.01); the CCO exercise of cells and the protein expression stage of ATP-sensitive potassium channel in sodium hydrosulfide+glibenclamide group ((1.79±0.06) nmol·min(-1)·mg(-1) and 0.74±0.07) was considerably decrease than these of sodium hydrosulfide solely group (P<0.05 or P<0.01).

Sodium hydrosulfide can enhance the survival fee of rat epidermal cells after the intervention of burn serum, which is expounded to the alleviation of epidermal cell mitochondrial injury and induced by ATP-sensitive potassium ion channel.

[Effects and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum]

Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP exercise and Runx2 protein expression

As a flavonoid, rutin has been discovered to have a variety of organic features, comparable to resisting irritation and oxidation, and stopping cerebral hemorrhage and hypertension. It has been discovered to play an necessary function in osteoporosis and different orthopedic illnesses lately. MC3T3-E1 cells have been randomly divided right into a management group, a rutin-1 group (0.01 mmol/L), a rutin-2 group (0.05 mmol/L) and a rutin-Three group (0.1 mmol/L). Osteogenic differentiation of cells was induced by osteogenic induction fluid. The management group was handled with the utmost dose of drug solvent. 2~Three days later, the solvent was changed with contemporary osteogenic induction fluid containing rutin.

After a sure interval of routine tradition, the cells have been collected for subsequent experiments. The expression of Runx2 gene in cells in all teams was detected by Real-time PCR; the expression of Runx2 protein was detected by Western blot and immunocytochemistry; the exercise of ALP was detected by reagent package technique; osteogenic differentiation was analyzed by alizarin purple staining. The outcomes of Real-time PCR confirmed that, in contrast with the management group, the therapy of cells with rutin can considerably enhance the expression of Runx2 gene (p<0.05); the upper the focus, the upper the expression of Runx2 gene, and important variations have been discovered amongst teams wherein totally different concentrations have been used (p<0.05); the outcomes of Western blot and IHC confirmed that the expression development of Runx2 protein in every group was in step with PCR outcomes.

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OOSA10411-5ML - SERUM:Azide Free Serum & Cells

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GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products

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OOSA10413-5ML - Serum:Azide Free

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Bovine Serum Albumin Fatty Acid Free Reagent Grade

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Guinea pig serum

IHR-8137 10 ml
EUR 47.75

Guinea Pig Serum

GPS 1 ml/vial
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abx810186-10nmol 10 nmol
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EUR 194.92

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Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

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EUR 1201.2

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Guinea Pig Serum - 50ml

GU-310/50 50ml
EUR 97.79

Normal Guinea Pig Serum

88-NP25 50 ml
EUR 270
Description: Normal Guinea Pig Serum

Normal Guinea Pig Serum

88R-1016 5 ml
EUR 122
Description: Normal Guinea Pig Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Guinea Pig Serum Albumin

30R-3306 10 mg
EUR 303
Description: Purified native Guinea Pig serum albumin

Guinea pig serum albumin

CSB-NP002001Gp 10mg Ask for price

Free Chlorine Reagents 0-5mg/L - EACH

WAT1028 EACH
EUR 49.95

Guinea Pig Complement Serum

32R-CP004 5 ml
EUR 447
Description: Purified Guinea Pig Complement adsorbed with mouse cells

Guinea Pig Complement Serum

32R-CP006 3 ml
EUR 98
Description: Purified Guinea Pig Complement isolated from the blood of normal Hartley guinea pigs

Guinea Pig Complement Serum

32R-CP007 25 ml
EUR 807.6
Description: Purified Guinea Pig Complement adsorbed with sheep red blood cells

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Non-sterile guinea pig serum

GPS05-0050 50 ml
EUR 277.68

Non-sterile guinea pig serum

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EUR 332.28

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OORA00623-5ML - GUINEA PIG SERUM

OORA00623-5ML 5mL
EUR 269

Guinea Pig Serum Albumin antibody

70R-15101 100 ug
EUR 302
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody

OORA00649-50ML - GUINEA PIG SERUM

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EUR 869

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Guinea pig Serum Iron ELISA kit

E05S0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Iron ELISA kit

E05S0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Iron ELISA kit

E05S0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Albumin, Lyophilized

22070080-1 50 mg
EUR 168.11

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EUR 728.41

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22070080-4 1000 mg
EUR 1147.51

Guinea Pig Serum Albumin ELISA kit

E01A42617 96T
EUR 700
Description: ELISA

Guinea pig Serum Albumin ELISA kit

E05S0014-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Albumin ELISA kit

E05S0014-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Albumin ELISA kit

E05S0014-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit

E05S0228-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit

E05S0228-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit

E05S0228-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Albumin antibody (HRP)

60R-1144 100 ug
EUR 302
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (HRP)

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60R-1145 100 ug
EUR 302
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (FITC)

Guinea Pig Serum Albumin antibody (biotin)

60R-1146 100 ug
EUR 302
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (biotin)

Guinea Pig Serum amyloid A ELISA kit

E01A42729 96T
EUR 700
Description: ELISA

Guinea Pig Serum amyloid A ELISA Kit

ELA-E0885Gu 96 Tests
EUR 1113.6

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Guinea Pig Serum Albumin Polyclonal Antibody

A57556
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  • 100 µg
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Guinea Pig control serum (non-immunized) Mixed breed, Mouse serum -ve & G. Pig +ve

NGPS-25-GM 25 ml
EUR 489.6

VIP guinea pig polyclonal antibody, Serum

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Guinea Pig Serum amyloid A 3 ELISA kit

E01A42730 96T
EUR 700
Description: ELISA

Guinea pig Serum amyloid A 3 ELISA kit

E05S0229-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid A 3 ELISA kit

E05S0229-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E05S0229-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig serum albumin antibody ELISA kit

E01A42787 96T
EUR 700
Description: ELISA

Guinea pig serum albumin antibody ELISA kit

E05S0304-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig serum albumin antibody ELISA kit

E05S0304-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig serum albumin antibody ELISA kit

E05S0304-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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IGGPSER1000ML each
EUR 12400
Description: Innovative Grade US Origin Guinea Pig Serum

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EUR 1395
Description: Innovative Grade US Origin Guinea Pig Serum

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EUR 163
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EUR 388
Description: Innovative Grade US Origin Guinea Pig Serum

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EUR 6588
Description: Innovative Grade US Origin Guinea Pig Serum

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EUR 737
Description: Innovative Grade US Origin Guinea Pig Serum

Sterile filtered guinea pig serum, 0.2 micron

SGP30-0050 50 ml
EUR 193.75

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EUR 256.25

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EUR 775

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SND1 guinea pig polyclonal antibody, Serum

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Guinea pig Serum Amyloid A(SAA) ELISA Kit

NSL0280Gp 96T
EUR 528

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Guinea pig Serum Amyloid A2 (SAA2) ELISA Kit

abx357760-96tests 96 tests
EUR 990

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BP5105 100 µl Ask for price

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Guinea pig Serum Response Factor (SRF) ELISA Kit

abx357768-96tests 96 tests
EUR 990

Guinea Pig Serum response factor(SRF) ELISA kit

E01A42879 96T
EUR 700
Description: ELISA

Guinea pig Serum response factor(SRF) ELISA kit

E05S0401-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum response factor(SRF) ELISA kit

E05S0401-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum response factor(SRF) ELISA kit

E05S0401-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Amyloid P Component ELISA kit

E01A42735 96T
EUR 700
Description: ELISA

Guinea pig Serum Amyloid P Component ELISA kit

E05S0234-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Amyloid P Component ELISA kit

E05S0234-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Amyloid P Component ELISA kit

E05S0234-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

LOC100653049 guinea pig polyclonal antibody, Serum

BP5085 100 µl Ask for price

GUINEA PIG SERUM COMPLEMENT, DOMESTIC, LIQUID, 100 ML

7243150-100ML 1ML
EUR 886.99

GUINEA PIG SERUM COMPLEMENT, DOMESTIC, LIQUID, 500 ML

7243150-500ML 1ML
EUR 3558.97

GUINEA PIG SERUM COMPLEMENT, DOMESTIC, LIQUID, 50 ML

7243150-50ML 1ML
EUR 553.18

Guinea Pig Glycosylated serum protein ELISA kit

E01A39754 96T
EUR 700
Description: ELISA

Guinea pig Glycosylated serum protein ELISA kit

E05G0373-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Glycosylated serum protein ELISA kit

E05G0373-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

In drug therapy teams, the exercise of ALP was considerably larger than that within the management group (p<0.05); there have been important variations amongst teams wherein totally different concentrations have been used (p<0.05).

The outcomes of alizarin purple staining confirmed that calcified nodules have been shaped in all teams and that the world of calcified nodules shaped in teams handled with rutin was higher than that within the management group; the higher the focus, the bigger the world. Rutin can promote osteoblastic differentiation; and the higher the focus, the more practical it’s.