Class II main histocompatibility advanced peptide (MHC-IIp) multimers are exactly engineered reagents used to detect T cells particular for antigens from pathogens, tumors, and self-proteins. While the associated Class I MHC/peptide (MHC-Ip) multimers are often produced from subunits expressed in E. coli, most Class II MHC alleles can’t be produced in micro organism, and this has contributed to the notion that MHC-IIp reagents are tougher to supply.
Herein, we current a sturdy constitutive expression system for soluble biotinylated MHC-IIp proteins that makes use of steady lentiviral vector-transduced derivatives of HEK-293T cells. The expression design contains allele-specific peptide ligands tethered to the amino-terminus of the MHC-II β chain by way of a protease-cleavable linker. Following cleavage of the linker, HLA-DM is used to catalyze environment friendly peptide alternate, enabling high-throughput manufacturing of many distinct MHC-IIp complexes from a single manufacturing cell line.
Peptide alternate is monitored utilizing both of two label-free strategies, native isoelectric focusing gel electrophoresis or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of eluted peptides.
Together, these strategies produce MHC-IIp complexes which might be extremely homogeneous and that type the premise for excellent MHC-IIp multimer reagents. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Lentivirus manufacturing and expression line creation Support Protocol 1: Six-well assay for estimation of manufacturing cell line yield Support Protocol 2: Universal ELISA for quantifying proteins with fused leucine zippers and His-tags Basic Protocol 2: Cultures for manufacturing of Class II MHC proteins Basic
Protocol 3: Purification of Class II MHC proteins by anti-leucine zipper affinity chromatography Alternate Protocol 1: IMAC purification of His-tagged Class II MHC Support Protocol 3: Protein focus measurements and changes Support Protocol 4: Polishing purification by anion-exchange chromatography
Support Protocol 5: Estimating biotinylation share by streptavidin precipitation Basic Protocol 4: Peptide alternate Basic Protocol 5: Analysis of peptide alternate by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Alternate Protocol 2: Native isoelectric focusing to validate MHC-II peptide loading Basic Protocol 6: Multimerization Basic Protocol 7: Staining cells with Class II MHC tetramers.
Autonomous spheroid formation by tradition plate compartmentation
Scaffold-free 3D cell cultures (e.g. pellet cultures) are broadly used in medical science, together with cartilage regeneration. Their drawbacks are excessive time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid era, most tradition methods stay costly or require tedious procedures. The purpose of this research was to ascertain a system for useful resource environment friendly spheroid era.
This was achieved by compartmentation of cell tradition surfaces utilising laser engraving (grid plates). This compartmentation triggered autonomous spheroid formation by way of rolling-up of the cell monolayer in human adipose-derived stem cells (ASC/TERT1) and human articular chondrocytes (hAC)-ASC/TERT1 co-cultures, when cultivated on grid plates below chondrogenic circumstances. Plates with Three mm grid dimension yielded steady diameters (about 300 μm). ASC/TERT1 spheroids totally shaped inside
Three weeks whereas co-cultures took 1-2 weeks, forming considerably quicker with rising hAC ratio (p<0.05 and 0.01 for 1:1 and 1:Four ASC/TERT1:hAC ratio respectively). Co-cultures confirmed barely decrease spheroid diameter, attributable to earlier spheroid formation and incomplete monolayer formation. However, this was related to extra common matrix distribution in the co-tradition.
Both confirmed differentiation capability comparable to plain pellet tradition in (immune-)histochemistry and RT-qPCR. To assess usability for cartilage restore, spheroids have been embedded right into a hydrogel (fibrin), yielding cellular outgrowth and matrix deposition, which was particularly pronounced in co-cultures. The herein offered novel cell tradition system is just not solely a promising instrument for autonomous spheroid era with the potential of experimental and scientific software in tissue engineering but in addition for high-throughput evaluation for each pharmaceutical and therapeutic makes use of.
Development of a novel β-1,6-glucan-specific detection system utilizing functionally-modified recombinant endo-β-1,6-glucanase
β-1,3D-Glucan is a ubiquitous glucose polymer produced by vegetation, micro organism, and most fungi. It has been used as a diagnostic instrument in sufferers with invasive mycoses by way of a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no methodology is presently obtainable for measuring β-1,6-glucan, one other main β-glucan construction of fungal polysaccharides.
Herein, we describe the event of a cheap and highly-sensitive and particular assay for β-1,6-glucan utilizing a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase exercise. The purified β-1,6-glucanase by-product certain to the β-1,6-glucan pustulan with a OkayD of 16.Four nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its capacity to detect cell wall β-1,6-glucan from each yeast and hyphal types of the opportunistic fungal pathogen Candida albicans, with none detectable binding to glucan missing the lengthy β-1,6-glucan department.
RABBIT SERUM WITH 0.09% AZIDE Serum Products |
GWB-Q00017 |
GenWay Biotech |
20 ml |
Ask for price |
RABBIT SERUM WITH 0.09% AZIDE Serum Products |
GWB-Q00018 |
GenWay Biotech |
100 ml |
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GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products |
GWB-Q00004 |
GenWay Biotech |
5 ml |
Ask for price |
OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells |
OOSA10429-10ML |
Aviva Systems Biology |
10ml |
EUR 279 |
|
OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells |
OOSA10433-100ML |
Aviva Systems Biology |
100ml |
EUR 1799 |
|
Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma |
EXOFLOW2-BASICA-SP |
SBI |
30 rxn |
EUR 1201.2 |
|
Goat Serum (10%) in PBST with Azide |
30611260-1 |
Glycomatrix |
100 ml |
EUR 33.41 |
Goat Serum (10%) in PBST with Azide |
30611260-2 |
Glycomatrix |
250 ml |
EUR 59.36 |
Horse Serum (10%) in PBST with Azide |
30611278-1 |
Glycomatrix |
100 ml |
EUR 31.76 |
Horse Serum (10%) in PBST with Azide |
30611278-2 |
Glycomatrix |
250 ml |
EUR 59.79 |
Goat Serum (10%) in TBST Buffer with Azide |
30611263-1 |
Glycomatrix |
100 mL |
EUR 33.41 |
Goat Serum (10%) in TBST Buffer with Azide |
30611263-2 |
Glycomatrix |
250 ml |
EUR 57.19 |
Horse Serum (10%) in PBS with Azide, pH 7.4 |
40120170-1 |
Glycomatrix |
100 ml |
EUR 31.76 |
Horse Serum (10%) in PBS with Azide, pH 7.4 |
40120170-2 |
Glycomatrix |
250 ml |
EUR 54.54 |
Goat Serum (10%) in TBST Wash Buffer with Azide |
30611266-1 |
Glycomatrix |
100 ml |
EUR 33.41 |
Goat Serum (10%) in TBST Wash Buffer with Azide |
30611266-2 |
Glycomatrix |
250 ml |
EUR 57.11 |
Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile |
30611251-1 |
Glycomatrix |
100 ml |
EUR 32.94 |
Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile |
30611251-2 |
Glycomatrix |
250 ml |
EUR 59.44 |
Horse Serum (10%) in TBST with Sodium Azide, Sterile |
30611281-1 |
Glycomatrix |
100 ml |
EUR 31.76 |
Horse Serum (10%) in TBST with Sodium Azide, Sterile |
30611281-2 |
Glycomatrix |
250 ml |
EUR 54.54 |
VEX Exosome Isolation Reagent (from serum) |
R602 |
Vazyme |
10 ml |
EUR 769.2 |
Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile |
30611257-1 |
Glycomatrix |
100 ml |
EUR 33.41 |
Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile |
30611257-2 |
Glycomatrix |
250 ml |
EUR 57.11 |
Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile |
30611275-1 |
Glycomatrix |
50 ml |
EUR 36.77 |
Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile |
30611275-2 |
Glycomatrix |
100 ml |
EUR 54.54 |
Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile |
30611254-1 |
Glycomatrix |
100 ml |
EUR 33.41 |
Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile |
30611254-2 |
Glycomatrix |
250 ml |
EUR 57.11 |
Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile |
30611272-1 |
Glycomatrix |
50 ml |
EUR 31.67 |
Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile |
30611272-2 |
Glycomatrix |
100 ml |
EUR 54.64 |
Bovine Serum Albumin Fatty Acid Free Reagent Grade |
GWB-F06E29 |
GenWay Biotech |
1 kg |
Ask for price |
T-Pro Total Exosome Isolation reagent (from serum) |
JO66-V002M |
T-Pro Biotechnology |
25ml/BT |
EUR 800 |
T-Pro Total Exosome Isolation reagent (from serum) |
JO66-V002S |
T-Pro Biotechnology |
1ml*5/set |
EUR 200 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
7917-100 |
Biovision |
each |
EUR 783.6 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
7917-25 |
Biovision |
each |
EUR 294 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
7917-5 |
Biovision |
each |
EUR 144 |
Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile |
30611283-1 |
Glycomatrix |
100 ml |
EUR 31.76 |
Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile |
30611283-2 |
Glycomatrix |
250 ml |
EUR 54.54 |
MOUSE SERUM:Azide Free Serum Products |
GWB-Q00010 |
GenWay Biotech |
5 ml |
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MOUSE SERUM:Azide Free Serum Products |
GWB-Q00011 |
GenWay Biotech |
10 ml |
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MOUSE SERUM:Azide Free Serum Products |
GWB-Q00012 |
GenWay Biotech |
50 ml |
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MOUSE SERUM:Azide Free Serum Products |
GWB-Q00013 |
GenWay Biotech |
100 ml |
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MOUSE SERUM:Azide Free Serum Products |
GWB-Q00014 |
GenWay Biotech |
500 ml |
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ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
CEIK-044L-30T |
Creative Bioarray |
30 T |
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ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
CEIK-044L-50T |
Creative Bioarray |
50 T |
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Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW! |
P5W13 |
101Bio |
10 rxn |
EUR 99 |
OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade |
OPEF01351-100G |
Aviva Systems Biology |
100g |
EUR 1302 |
|
Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade |
GWB-DCC190 |
GenWay Biotech |
1 kg |
Ask for price |
MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml) |
WA-013 |
Inventbiotech |
each |
EUR 135 |
Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: SPM376 |
APR07282G |
Leading Biology |
0.05mg |
EUR 475.2 |
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E |
Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: ALB/398 |
APR07283G |
Leading Biology |
0.05mg |
EUR 475.2 |
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E |
MOUSE SERUM Serum Products |
GWB-Q00006 |
GenWay Biotech |
100 ml |
Ask for price |
EZ-DNA Reagents |
BS8202 |
Bio Basic |
100preps |
EUR 105.67 |
|
EZ-DNA Reagents |
SK8201 |
Bio Basic |
100preps |
EUR 112.2 |
|
DOG SERUM:Azide free Serum Products |
GWB-Q00003 |
GenWay Biotech |
100 ml |
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RAT SERUM:Azide Free Serum Products |
GWB-Q00024 |
GenWay Biotech |
10 ml |
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RAT SERUM:Azide Free Serum Products |
GWB-Q00025 |
GenWay Biotech |
50 ml |
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RAT SERUM:Azide Free Serum Products |
GWB-Q00026 |
GenWay Biotech |
100 ml |
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RABBIT SERUM:Azide Free Serum Products |
GWB-Q00019 |
GenWay Biotech |
20 ml |
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RABBIT SERUM:Azide Free Serum Products |
GWB-Q00020 |
GenWay Biotech |
100 ml |
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Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder |
20009-WA-1 |
Alpha Diagnostics |
1 mg |
EUR 169.2 |
Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder |
20009-WA-5 |
Alpha Diagnostics |
5 mg |
EUR 343.2 |
Mouse Serum |
abx098261-100L |
Abbexa |
100 L |
EUR 155407.2 |
|
Mouse Serum |
20-abx098261 |
Abbexa |
-
Ask for price
-
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|
|
|
Mouse Serum |
abx098261-5L |
Abbexa |
5 L |
EUR 10599.6 |
|
Mouse Serum |
abx098262-1ml |
Abbexa |
1 ml |
EUR 2064 |
|
Mouse Serum |
abx098261-1ml |
Abbexa |
1 ml |
Ask for price |
Mouse Serum |
abx098261-200l |
Abbexa |
200 µl |
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Mouse Serum |
M2021-050 |
GenDepot |
500ml |
EUR 970 |
Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile |
30611269-1 |
Glycomatrix |
50 mL |
EUR 36.77 |
Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile |
30611269-2 |
Glycomatrix |
100 ml |
EUR 57.64 |
GUINEA PIG SERUM:Azide Free Serum Products |
GWB-Q00005 |
GenWay Biotech |
5 ml |
Ask for price |
Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: SPM376 |
APR07284G |
Leading Biology |
0.1mg |
EUR 580.8 |
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E |
Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: ALB/398 |
AMM04382G |
Leading Biology |
0.1mg |
EUR 580.8 |
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E |
CryoProtX Mix Reagents 1.5 mL |
M-MDSR-61 |
MiTeGen |
1.5 ml ml |
EUR 37 |
Description: CryoProtX Mix Reagents 1.5 mL |
Mouse Serum Amyloid P Component (SAP) Reference serum |
SAP12-RS |
Alpha Diagnostics |
100 ul |
EUR 388.8 |
Human IgG (serum origin, purified >97%, low endotoxin, azide free) |
20007-1-LE-1 |
Alpha Diagnostics |
1 mg |
EUR 196.8 |
Set of 10 Biolipidure Reagents |
Biolipidure-set |
AS ONE International |
10mLx10 |
EUR 1820.4 |
|
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Normal Mouse Serum |
88-NM35 |
Fitzgerald |
50 ml |
EUR 459 |
|
Description: Normal Mouse Serum |
Normal Mouse Serum |
88R-1002 |
Fitzgerald |
5 ml |
EUR 118 |
|
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Normal Mouse Serum |
BMS0070-10mL |
Abbkine |
10 mL |
EUR 89 |
Description: High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays. |
Normal Mouse Serum |
BMS0070-10mL10 |
Abbkine |
10 mL×10 |
EUR 529 |
Description: High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays. |
Normal Mouse Serum |
BMS0070-each |
Abbkine |
each |
Ask for price |
Mouse Serum Albumin |
30R-3304 |
Fitzgerald |
10 mg |
EUR 153 |
|
Description: Purified native Mouse Serum Albumin |
Mouse Serum Albumin |
CSB-NP000801m |
Cusabio |
10mg |
Ask for price |
CryoProtX Mix Eco Reagents 1.5 mL |
M-MDSR-61-ECO |
MiTeGen |
1.5 ml ml |
EUR 37 |
Description: CryoProtX Mix Eco Reagents 1.5 mL |
PeliKine tool set, additional reagents |
M1980 |
Tecan Benelux |
1 unit |
EUR 169.8 |
Mouse Complement Serum |
32R-CM001 |
Fitzgerald |
5 ml |
EUR 526 |
|
Description: Frozen high activity mouse complement serum |
VACUETTE Bleeding with Gel Activator Serum 8ml Greiner VACUETTE Bleeding with Gel Activator Serum 8ml Greiner - PK50 |
455071A |
Scientific Laboratory Supplies |
PK50 |
EUR 62.1 |
Mouse Balb C Serum |
IMSBCSER1000ML |
Innovative research |
each |
EUR 2265 |
|
Description: Mouse Balb C Serum |
Mouse Balb C Serum |
IMSBCSER100ML |
Innovative research |
each |
EUR 368 |
|
Description: Mouse Balb C Serum |
Mouse Balb C Serum |
IMSBCSER10ML |
Innovative research |
each |
EUR 70 |
|
Description: Mouse Balb C Serum |
Mouse Balb C Serum |
IMSBCSER25ML |
Innovative research |
each |
EUR 155 |
|
Description: Mouse Balb C Serum |
Mouse Balb C Serum |
IMSBCSER500ML |
Innovative research |
each |
EUR 1199 |
|
Description: Mouse Balb C Serum |
Mouse Balb C Serum |
IMSBCSER50ML |
Innovative research |
each |
EUR 187 |
|
Description: Mouse Balb C Serum |
Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free) |
20007-1-LE-BTN |
Alpha Diagnostics |
100 ug |
EUR 343.2 |
Mouse Serum albumin (Alb) |
1-CSB-YP001561MO |
Cusabio |
-
Ask for price
-
Ask for price
-
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-
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-
Ask for price
-
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|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast |
Mouse Serum albumin (Alb) |
1-CSB-BP001561MO |
Cusabio |
-
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-
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-
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-
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-
Ask for price
-
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|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus |
Non-sterile mouse serum |
MS05-0050 |
Equitech |
50 ml |
EUR 156.25 |
|
Non-sterile mouse serum |
MS05-0100 |
Equitech |
100 ml |
EUR 188.75 |
|
Non-sterile mouse serum |
MS05-0500 |
Equitech |
500 ml |
EUR 531.25 |
|
*Mouse IgG Depleted Serum |
IMSIGGDS100ML |
Innovative research |
each |
EUR 3730 |
|
Description: *Mouse IgG Depleted Serum |
*Mouse IgG Depleted Serum |
IMSIGGDS10ML |
Innovative research |
each |
EUR 466 |
|
Description: *Mouse IgG Depleted Serum |
Mouse Serum Albumin protein |
30-1179 |
Fitzgerald |
10 mg |
EUR 234 |
|
Description: Purified native Mouse Albumin protein |
Mouse Serum Albumin Protein |
20-abx600021 |
Abbexa |
-
Ask for price
-
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-
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-
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|
|
|
Normal Mouse Serum Filtered |
GWB-E7645B |
GenWay Biotech |
10 ml |
Ask for price |
Mouse Serum Albumin Protein |
abx600021-100g |
Abbexa |
100 µg |
EUR 237.5 |
Mouse Serum Albumin Protein |
abx600021-200g |
Abbexa |
200 µg |
EUR 837.5 |
Mouse Serum Albumin Protein |
abx600021-50g |
Abbexa |
50 µg |
EUR 175 |
Mouse Serum Albumin antibody |
20R-AR005 |
Fitzgerald |
20 mg |
EUR 387 |
|
Description: Rabbit polyclonal Mouse Serum Albumin antibody |
Mouse Serum albumin antibody |
20R-AR041 |
Fitzgerald |
2 ml |
EUR 343 |
|
Description: Rabbit polyclonal Mouse Serum albumin antibody |
Mouse Serum Albumin antibody |
70R-AG001 |
Fitzgerald |
1 mg |
EUR 209 |
|
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody |
Mouse Serum Lipid-Stripped |
GWB-D35A77 |
GenWay Biotech |
1 l |
Ask for price |
WASH SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN |
99-784-CM |
CORNING |
1 L /pk |
EUR 85.2 |
Description: Specialty Media; Islet products |
Bradford(Coomassie) Protein Assay Plus Reagents |
P7201-050 |
GenDepot |
450ml |
EUR 225.6 |
Bradford(Coomassie) Protein Assay Plus Reagents |
P7201-100 |
GenDepot |
950ml |
EUR 130 |
Mouse Normal Serum, Antibody |
GWB-9A9CFB |
GenWay Biotech |
0.05 ml |
Ask for price |
Mouse Serum Albumin, Lyophilized |
22070104-1 |
Glycomatrix |
100 mg |
EUR 261.45 |
Mouse Serum Albumin, Lyophilized |
22070104-2 |
Glycomatrix |
500 mg |
EUR 822.66 |
Mouse Serum Albumin, Lyophilized |
22070104-3 |
Glycomatrix |
1000 mg |
EUR 1280.2 |
DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN |
99-783-CM |
CORNING |
1 L /pk |
EUR 75.6 |
Description: Specialty Media; Islet products |
Mouse Serum Iron ELISA kit |
E03S0273-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum Iron ELISA kit |
E03S0273-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum Iron ELISA kit |
E03S0273-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Liposomal and Non-Liposomal Transfection Reagents |
abx298032-100g |
Abbexa |
100 µg |
Ask for price |
Liposomal and Non-Liposomal Transfection Reagents |
abx298032-20g |
Abbexa |
20 µg |
EUR 875 |
Liposomal and Non-Liposomal Transfection Reagents |
abx298032-50g |
Abbexa |
50 µg |
Ask for price |
Serum Amyloid A Mouse mAb |
E2222727 |
EnoGene |
100ul |
EUR 255 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
Mouse Serum Albumin ELISA kit |
E01A25178 |
BlueGene |
96T |
EUR 700 |
Description: ELISA |
Mouse Serum Albumin ELISA kit |
E03S0014-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum Albumin ELISA kit |
E03S0014-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum Albumin ELISA kit |
E03S0014-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum amyloid ELISA kit |
E03S0228-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum amyloid ELISA kit |
E03S0228-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Serum amyloid ELISA kit |
E03S0228-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Fetal Bovine Serum(Without Exosome ) |
FBSNE-01061 |
Cyagen |
20/50mL |
Ask for price |
We developed a sandwich ELISA-like assay with a low restrict of quantification for pustulan (1.5 pg/ml), and we efficiently employed this assay in the quantification of furthercellular β-1,6-glucan launched by >250 patient-derived strains of completely different Candida species (together with Candida auris) in tradition supernatant in vitro. We additionally used this assay to measure β-1,6-glucan in vivo in the serum and in a number of organs in a mouse mannequin of systemic candidiasis. Our work describes a dependable methodology for β-1,6-glucan detection, which can show helpful for the prognosis of invasive fungal infections.