Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Class II main histocompatibility advanced peptide (MHC-IIp) multimers are exactly engineered reagents used to detect T cells particular for antigens from pathogens, tumors, and self-proteins. While the associated Class I MHC/peptide (MHC-Ip) multimers are often produced from subunits expressed in E. coli, most Class II MHC alleles can’t be produced in micro organism, and this has contributed to the notion that MHC-IIp reagents are tougher to supply.

Herein, we current a sturdy constitutive expression system for soluble biotinylated MHC-IIp proteins that makes use of steady lentiviral vector-transduced derivatives of HEK-293T cells. The expression design contains allele-specific peptide ligands tethered to the amino-terminus of the MHC-II β chain by way of a protease-cleavable linker. Following cleavage of the linker, HLA-DM is used to catalyze environment friendly peptide alternate, enabling high-throughput manufacturing of many distinct MHC-IIp complexes from a single manufacturing cell line.

Peptide alternate is monitored utilizing both of two label-free strategies, native isoelectric focusing gel electrophoresis or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of eluted peptides.

Together, these strategies produce MHC-IIp complexes which might be extremely homogeneous and that type the premise for excellent MHC-IIp multimer reagents. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Lentivirus manufacturing and expression line creation Support Protocol 1: Six-well assay for estimation of manufacturing cell line yield Support Protocol 2: Universal ELISA for quantifying proteins with fused leucine zippers and His-tags Basic Protocol 2: Cultures for manufacturing of Class II MHC proteins Basic

Protocol 3: Purification of Class II MHC proteins by anti-leucine zipper affinity chromatography Alternate Protocol 1: IMAC purification of His-tagged Class II MHC Support Protocol 3: Protein focus measurements and changes Support Protocol 4: Polishing purification by anion-exchange chromatography

Support Protocol 5: Estimating biotinylation share by streptavidin precipitation Basic Protocol 4: Peptide alternate Basic Protocol 5: Analysis of peptide alternate by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Alternate Protocol 2: Native isoelectric focusing to validate MHC-II peptide loading Basic Protocol 6: Multimerization Basic Protocol 7: Staining cells with Class II MHC tetramers.

Autonomous spheroid formation by tradition plate compartmentation

Scaffold-free 3D cell cultures (e.g. pellet cultures) are broadly used in medical science, together with cartilage regeneration. Their drawbacks are excessive time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid era, most tradition methods stay costly or require tedious procedures. The purpose of this research was to ascertain a system for useful resource environment friendly spheroid era.

This was achieved by compartmentation of cell tradition surfaces utilising laser engraving (grid plates). This compartmentation triggered autonomous spheroid formation by way of rolling-up of the cell monolayer in human adipose-derived stem cells (ASC/TERT1) and human articular chondrocytes (hAC)-ASC/TERT1 co-cultures, when cultivated on grid plates below chondrogenic circumstances. Plates with Three mm grid dimension yielded steady diameters (about 300 μm). ASC/TERT1 spheroids totally shaped inside

Three weeks whereas co-cultures took 1-2 weeks, forming considerably quicker with rising hAC ratio (p<0.05 and 0.01 for 1:1 and 1:Four ASC/TERT1:hAC ratio respectively). Co-cultures confirmed barely decrease spheroid diameter, attributable to earlier spheroid formation and incomplete monolayer formation. However, this was related to extra common matrix distribution in the co-tradition.

Both confirmed differentiation capability comparable to plain pellet tradition in (immune-)histochemistry and RT-qPCR. To assess usability for cartilage restore, spheroids have been embedded right into a hydrogel (fibrin), yielding cellular outgrowth and matrix deposition, which was particularly pronounced in co-cultures. The herein offered novel cell tradition system is just not solely a promising instrument for autonomous spheroid era with the potential of experimental and scientific software in tissue engineering but in addition for high-throughput evaluation for each pharmaceutical and therapeutic makes use of.

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Development of a novel β-1,6-glucan-specific detection system utilizing functionally-modified recombinant endo-β-1,6-glucanase

β-1,3D-Glucan is a ubiquitous glucose polymer produced by vegetation, micro organism, and most fungi. It has been used as a diagnostic instrument in sufferers with invasive mycoses by way of a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no methodology is presently obtainable for measuring β-1,6-glucan, one other main β-glucan construction of fungal polysaccharides.

Herein, we describe the event of a cheap and highly-sensitive and particular assay for β-1,6-glucan utilizing a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase exercise. The purified β-1,6-glucanase by-product certain to the β-1,6-glucan pustulan with a OkayD of 16.Four nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its capacity to detect cell wall β-1,6-glucan from each yeast and hyphal types of the opportunistic fungal pathogen Candida albicans, with none detectable binding to glucan missing the lengthy β-1,6-glucan department.

RABBIT SERUM WITH 0.09% AZIDE Serum Products

GWB-Q00017 20 ml Ask for price

RABBIT SERUM WITH 0.09% AZIDE Serum Products

GWB-Q00018 100 ml Ask for price

GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products

GWB-Q00004 5 ml Ask for price

OOSA10412-5ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10412-5ML 5ml
EUR 69

OOSA10414-10ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10414-10ML 10ml
EUR 149

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10429-10ML 10ml
EUR 279

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10433-100ML 100ml
EUR 1799

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP 30 rxn
EUR 1201.2

Goat Serum (10%) in PBST with Azide

30611260-1 100 ml
EUR 33.41

Goat Serum (10%) in PBST with Azide

30611260-2 250 ml
EUR 59.36

Horse Serum (10%) in PBST with Azide

30611278-1 100 ml
EUR 31.76

Horse Serum (10%) in PBST with Azide

30611278-2 250 ml
EUR 59.79

Goat Serum (10%) in TBST Buffer with Azide

30611263-1 100 mL
EUR 33.41

Goat Serum (10%) in TBST Buffer with Azide

30611263-2 250 ml
EUR 57.19

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-1 100 ml
EUR 31.76

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-2 250 ml
EUR 54.54

Goat Serum (10%) in TBST Wash Buffer with Azide

30611266-1 100 ml
EUR 33.41

Goat Serum (10%) in TBST Wash Buffer with Azide

30611266-2 250 ml
EUR 57.11

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile

30611251-1 100 ml
EUR 32.94

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile

30611251-2 250 ml
EUR 59.44

Horse Serum (10%) in TBST with Sodium Azide, Sterile

30611281-1 100 ml
EUR 31.76

Horse Serum (10%) in TBST with Sodium Azide, Sterile

30611281-2 250 ml
EUR 54.54

VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 769.2

Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile

30611257-1 100 ml
EUR 33.41

Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile

30611257-2 250 ml
EUR 57.11

Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile

30611275-1 50 ml
EUR 36.77

Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile

30611275-2 100 ml
EUR 54.54

Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile

30611254-1 100 ml
EUR 33.41

Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile

30611254-2 250 ml
EUR 57.11

Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile

30611272-1 50 ml
EUR 31.67

Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile

30611272-2 100 ml
EUR 54.64

Bovine Serum Albumin Fatty Acid Free Reagent Grade

GWB-F06E29 1 kg Ask for price

T-Pro Total Exosome Isolation reagent (from serum)

JO66-V002M 25ml/BT
EUR 800

T-Pro Total Exosome Isolation reagent (from serum)

JO66-V002S 1ml*5/set
EUR 200

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-5 each
EUR 144

Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile

30611283-1 100 ml
EUR 31.76

Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile

30611283-2 250 ml
EUR 54.54

MOUSE SERUM:Azide Free Serum Products

GWB-Q00010 5 ml Ask for price

MOUSE SERUM:Azide Free Serum Products

GWB-Q00011 10 ml Ask for price

MOUSE SERUM:Azide Free Serum Products

GWB-Q00012 50 ml Ask for price

MOUSE SERUM:Azide Free Serum Products

GWB-Q00013 100 ml Ask for price

MOUSE SERUM:Azide Free Serum Products

GWB-Q00014 500 ml Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-50T 50 T Ask for price

Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!

P5W13 10 rxn
EUR 99

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade

OPEF01351-100G 100g
EUR 1302

Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade

GWB-DCC190 1 kg Ask for price

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)

WA-013 each
EUR 135

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: SPM376

APR07282G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: ALB/398

APR07283G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

MOUSE SERUM Serum Products

GWB-Q00006 100 ml Ask for price

EZ-DNA Reagents

BS8202 100preps
EUR 105.67

EZ-DNA Reagents

SK8201 100preps
EUR 112.2

DOG SERUM:Azide free Serum Products

GWB-Q00003 100 ml Ask for price

RAT SERUM:Azide Free Serum Products

GWB-Q00024 10 ml Ask for price

RAT SERUM:Azide Free Serum Products

GWB-Q00025 50 ml Ask for price

RAT SERUM:Azide Free Serum Products

GWB-Q00026 100 ml Ask for price

RABBIT SERUM:Azide Free Serum Products

GWB-Q00019 20 ml Ask for price

RABBIT SERUM:Azide Free Serum Products

GWB-Q00020 100 ml Ask for price

Ecl Detection Reagents

RPN3004 EACH
EUR 552.9

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-1 1 mg
EUR 169.2

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-5 5 mg
EUR 343.2

Mouse Serum

abx098261-100L 100 L
EUR 155407.2

Mouse Serum

20-abx098261
  • Ask for price
  • Ask for price
  • 100 ml
  • 10 ml

Mouse Serum

abx098261-5L 5 L
EUR 10599.6

Mouse Serum

abx098262-1ml 1 ml
EUR 2064

Mouse Serum

abx098261-1ml 1 ml Ask for price

Mouse Serum

abx098261-200l 200 µl Ask for price

Mouse serum

IHR-8141 10 ml
EUR 47.75

Mouse Serum

M2021-050 500ml
EUR 970

Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile

30611269-1 50 mL
EUR 36.77

Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile

30611269-2 100 ml
EUR 57.64

Mouse Serum - 100ml

MO-340/100 100ml
EUR 183.32

Mouse Serum - 500ml

MO-340/500 500ml
EUR 733.32

GUINEA PIG SERUM:Azide Free Serum Products

GWB-Q00005 5 ml Ask for price

Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: SPM376

APR07284G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E

OOSA10411-5ML - SERUM:Azide Free Serum & Cells

OOSA10411-5ML 5ml
EUR 99

Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: ALB/398

AMM04382G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

OOSA10417-50ML - SERUM:Azide Free Serum & Cells

OOSA10417-50ML 50ml
EUR 469

OOSA10426-20ML - SERUM:Azide Free Serum & Cells

OOSA10426-20ML 20ml
EUR 99

OOSA10430-10ML - SERUM:Azide Free Serum & Cells

OOSA10430-10ML 10ml
EUR 379

OOSA10419-100ML - SERUM:Azide Free Serum & Cells

OOSA10419-100ML 100ml
EUR 749

OOSA11794-100ML - SERUM:Azide free Serum & Cells

OOSA11794-100ML 100ml
EUR 1489

Ecl Detection Reagents - EACH

RPN2105 EACH
EUR 815.4

Mouse Serum Proteins goat polyclonal antibody, Serum

AP31483SU-N 1 ml Ask for price

Mouse Serum Proteins rabbit polyclonal antibody, Serum

AP31472SU-N 1 ml Ask for price

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Mouse Serum Amyloid P Component (SAP) Reference serum

SAP12-RS 100 ul
EUR 388.8

Mouse Serum - 10ml

MO-340/10 10ml
EUR 28.11

Mouse Serum - 20ml

MO-340/20 20ml
EUR 55

Mouse Serum - 50ml

MO-340/50 50ml
EUR 97.79

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 196.8

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Normal Mouse Serum

88-NM35 50 ml
EUR 459
Description: Normal Mouse Serum

Normal Mouse Serum

88R-1002 5 ml
EUR 118
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Mouse Serum

BMS0070-10mL 10 mL
EUR 89
Description: High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays.

Normal Mouse Serum

BMS0070-10mL10 10 mL×10
EUR 529
Description: High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays.

Normal Mouse Serum

NMS 1 ml/vial
EUR 91

Normal Mouse Serum

BMS0070-each each Ask for price

Mouse Serum Albumin

30R-3304 10 mg
EUR 153
Description: Purified native Mouse Serum Albumin

Mouse Serum Albumin

CSB-NP000801m 10mg Ask for price

REAGENTS HIGH RANGE AMMONIA - PK25

HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH

HI9373301 EACH
EUR 130.95

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

PeliKine tool set, additional reagents

M1980 1 unit
EUR 169.8

Mouse Complement Serum

32R-CM001 5 ml
EUR 526
Description: Frozen high activity mouse complement serum

Alkphos Direct Labelling Reagents - EACH

RPN3680 EACH
EUR 915.3

VACUETTE Bleeding with Gel Activator Serum 8ml Greiner VACUETTE Bleeding with Gel Activator Serum 8ml Greiner - PK50

455071A PK50
EUR 62.1

Mouse Balb C Serum

IMSBCSER1000ML each
EUR 2265
Description: Mouse Balb C Serum

Mouse Balb C Serum

IMSBCSER100ML each
EUR 368
Description: Mouse Balb C Serum

Mouse Balb C Serum

IMSBCSER10ML each
EUR 70
Description: Mouse Balb C Serum

Mouse Balb C Serum

IMSBCSER25ML each
EUR 155
Description: Mouse Balb C Serum

Mouse Balb C Serum

IMSBCSER500ML each
EUR 1199
Description: Mouse Balb C Serum

Mouse Balb C Serum

IMSBCSER50ML each
EUR 187
Description: Mouse Balb C Serum

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 343.2

Mouse Serum albumin (Alb)

1-CSB-YP001561MO
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast

Mouse Serum albumin (Alb)

1-CSB-BP001561MO
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus

Free Chlorine Reagents 0-5mg/L - EACH

WAT1028 EACH
EUR 49.95

Non-sterile mouse serum

MS05-0050 50 ml
EUR 156.25

Non-sterile mouse serum

MS05-0100 100 ml
EUR 188.75

Non-sterile mouse serum

MS05-0500 500 ml
EUR 531.25

*Mouse IgG Depleted Serum

IMSIGGDS100ML each
EUR 3730
Description: *Mouse IgG Depleted Serum

*Mouse IgG Depleted Serum

IMSIGGDS10ML each
EUR 466
Description: *Mouse IgG Depleted Serum

Mouse Serum Albumin protein

30-1179 10 mg
EUR 234
Description: Purified native Mouse Albumin protein

Mouse Serum Albumin Protein

20-abx600021
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 mg
  • 10 mg
  • 1 g
  • 1 mg

Normal Mouse Serum Filtered

GWB-E7645B 10 ml Ask for price

Mouse Serum Albumin Protein

abx600021-100g 100 µg
EUR 237.5

Mouse Serum Albumin Protein

abx600021-200g 200 µg
EUR 837.5

Mouse Serum Albumin Protein

abx600021-50g 50 µg
EUR 175

Mouse Serum Albumin antibody

20R-AR005 20 mg
EUR 387
Description: Rabbit polyclonal Mouse Serum Albumin antibody

Mouse Serum albumin antibody

20R-AR041 2 ml
EUR 343
Description: Rabbit polyclonal Mouse Serum albumin antibody

Mouse Serum Albumin antibody

70R-AG001 1 mg
EUR 209
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody

Mouse Serum Lipid-Stripped

GWB-D35A77 1 l Ask for price

WASH SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN

99-784-CM 1 L /pk
EUR 85.2
Description: Specialty Media; Islet products

OOSA10418-100ML - SERUM Serum & Cells

OOSA10418-100ML 100ml
EUR 709

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 450ml
EUR 225.6

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-100 950ml
EUR 130

Mouse Normal Serum, Antibody

GWB-9A9CFB 0.05 ml Ask for price

Mouse Serum Albumin, Lyophilized

22070104-1 100 mg
EUR 261.45

Mouse Serum Albumin, Lyophilized

22070104-2 500 mg
EUR 822.66

Mouse Serum Albumin, Lyophilized

22070104-3 1000 mg
EUR 1280.2

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN

99-783-CM 1 L /pk
EUR 75.6
Description: Specialty Media; Islet products

Mouse Serum Iron ELISA kit

E03S0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Iron ELISA kit

E03S0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Iron ELISA kit

E03S0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Liposomal and Non-Liposomal Transfection Reagents

abx298032-100g 100 µg Ask for price

Liposomal and Non-Liposomal Transfection Reagents

abx298032-20g 20 µg
EUR 875

Liposomal and Non-Liposomal Transfection Reagents

abx298032-50g 50 µg Ask for price

Mouse Serum Amyloid P ELISA

KT-347 1 kit
EUR 551

Mouse Serum Iron (SIRON) ELISA

KT-58966 96 tests
EUR 981

Mouse control serum, CD-1

NMOS-CD1-1 1 ml
EUR 123.6

Mouse control serum, CD-1

NMOS-CD1-5 5 ml
EUR 196.8

Serum Amyloid A Mouse mAb

E2222727 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Mouse Serum Albumin ELISA kit

E01A25178 96T
EUR 700
Description: ELISA

Mouse Serum Albumin ELISA kit

E03S0014-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Albumin ELISA kit

E03S0014-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Albumin ELISA kit

E03S0014-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum amyloid ELISA kit

E03S0228-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum amyloid ELISA kit

E03S0228-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum amyloid ELISA kit

E03S0228-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Fetal Bovine Serum(Without Exosome )

FBSNE-01061 20/50mL Ask for price

Mouse control serum, Balb/c

NMOS-5BS 5 ml
EUR 196.8

We developed a sandwich ELISA-like assay with a low restrict of quantification for pustulan (1.5 pg/ml), and we efficiently employed this assay in the quantification of furthercellular β-1,6-glucan launched by >250 patient-derived strains of completely different Candida species (together with Candida auris) in tradition supernatant in vitro. We additionally used this assay to measure β-1,6-glucan in vivo in the serum and in a number of organs in a mouse mannequin of systemic candidiasis. Our work describes a dependable methodology for β-1,6-glucan detection, which can show helpful for the prognosis of invasive fungal infections.

Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line

A difficult facet with the use of protein hydrolysates in business manufacturing processes of recombinant therapeutic proteins is their impacts on the protein production resulting from a lack of understanding of batch-to-batch variability. Soy hydrolysates variability and its influence on fed-batch production of a recombinant monoclonal antibody (mAb) expressed in Sp2/zero cells have been studied utilizing 37 batches from the identical vendor.
The batch-to-batch variability of soy hydrolysates impacted cell development, titer and product high quality. Physico-chemical characterization of batches confirmed that soy hydrolysates are primarily a supply of amino acids and peptides containing decrease quantities of different parts corresponding to carbohydrates and chemical parts in cell tradition media. Soy hydrolysates composition of completely different batches was constant apart from hint parts. Statistical analyses recognized iron as a potential marker of a poor course of efficiency.
To confirm this correlation, two varieties of iron, ferric ammonium citrate and ferrous sulfate, have been added to a batch of soy hydrolysates related to a low stage of iron throughout cell tradition. Both varieties of iron decreased considerably cell development, mAb titer and elevated stage of the acidic cost variants of the mAb. Consequently, hint factor composition of soy hydrolysates or of all incoming uncooked supplies may result in important impacts on course of efficiency and product high quality and due to this fact must be tightly managed.

Mitochondrial dysfunction and oxidative stress in neurodegenerative illnesses.

Many strains of proof counsel that mitochondria have a central function in ageing-related neurodegenerative illnesses. Mitochondria are vital regulators of cell loss of life, a key characteristic of neurodegeneration. Mutations in mitochondrial DNA and oxidative stress each contribute to ageing, which is the best threat issue for neurodegenerative illnesses. In all main examples of these illnesses there may be sturdy proof that mitochondrial dysfunction happens early and acts causally in illness pathogenesis.
Moreover, a formidable quantity of disease-specific proteins work together with mitochondria. Thus, therapies concentrating on primary mitochondrial processes, corresponding to vitality metabolism or free-radical era, or particular interactions of disease-related proteins with mitochondria, maintain nice promise.

Glioblastoma microvesicles transport RNA and proteins that promote tumour development and present diagnostic biomarkers.

Glioblastoma tumour cells launch microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by regular host cells, corresponding to mind microvascular endothelial cells. By incorporating an mRNA for a reporter protein into these microvesicles, we display that messages delivered by microvesicles are translated by recipient cells. These microvesicles are additionally enriched in angiogenic proteins and stimulate tubule formation by endothelial cells. Tumour-derived microvesicles due to this fact function a means of delivering genetic data and proteins to recipient cells within the tumour surroundings.
Glioblastoma microvesicles additionally stimulated proliferation of a human glioma cell line, indicating a self-promoting facet. Messenger RNA mutant/variants and miRNAs attribute of gliomas could possibly be detected in serum microvesicles of glioblastoma sufferers. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma sufferers. Thus, tumour-derived microvesicles could present diagnostic data and help in therapeutic choices for most cancers sufferers by way of a blood take a look at.

Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic standards.

Endothelial cells have been remoted from freshly obtained human umbilical cords by collagenase digestion of the inside of the umbilical vein. The cells have been grown in tissue tradition as a homogeneous inhabitants for durations as much as 5 mo and some strains have been subcultured for 10 serial passages. During the logarithmic section of cell development, cell-doubling time was 92 h. Light, section distinction, and scanning electron microscopy demonstrated that cultured human endothelial cells grew as monolayers of intently opposed, polygonal massive cells whereas each cultured human fibroblasts and human easy muscle cells grew as overlapping layers of parallel arrays of slender, spindle-shaped cells.
By transmission electron microscopy, cultured endothelial cells have been seen to comprise cytoplasmic inclusions (Weibel-Palade our bodies) attribute of in situ endothelial cells. These inclusions have been additionally present in endothelial cells lining umbilical veins however weren’t seen in easy muscle cells or fibroblasts in tradition or in situ. Cultured endothelial cells contained ample portions of easy muscle actomyosin. Cultured endothelial cells additionally contained ABH antigens applicable to the tissue donor’s blood kind; these antigens weren’t detectable on cultured easy muscle cells or fibroblasts. These research display that it’s attainable to tradition morphologically and immunologically identifiable human endothelial cells for durations as much as 5 mo.

Generation of germline-competent induced pluripotent stem cells.

We have beforehand proven that pluripotent stem cells will be induced from mouse fibroblasts by retroviral introduction of Oct3/4 (additionally referred to as Pou5f1), Sox2, c-Myc and Klf4, and subsequent choice for Fbx15 (additionally referred to as Fbxo15) expression. These induced pluripotent stem (iPS) cells (hereafter referred to as Fbx15 iPS cells) are much like embryonic stem (ES) cells in morphology, proliferation and teratoma formation; nonetheless, they’re completely different almost about gene expression and DNA methylation patterns, and fail to supply grownup chimaeras.
Here we present that choice for Nanog expression leads to germline-competent iPS cells with elevated ES-cell-like gene expression and DNA methylation patterns in contrast with Fbx15 iPS cells. The 4 transgenes (Oct3/4, Sox2, c-myc and Klf4) have been strongly silenced in Nanog iPS cells. We obtained grownup chimaeras from seven Nanog iPS cell clones, with one clone being transmitted by way of the germ line to the subsequent era.
Product not found
Approximately 20% of the offspring developed tumours attributable to reactivation of the c-myc transgene. Thus, iPS cells competent for germline chimaeras will be obtained from fibroblasts, however retroviral introduction of c-Myc ought to be averted for medical utility.