Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Class II main histocompatibility advanced peptide (MHC-IIp) multimers are exactly engineered reagents used to detect T cells particular for antigens from pathogens, tumors, and self-proteins. While the associated Class I MHC/peptide (MHC-Ip) multimers are often produced from subunits expressed in E. coli, most Class II MHC alleles can’t be produced in micro organism, and this has contributed to the notion that MHC-IIp reagents are tougher to supply.

Herein, we current a sturdy constitutive expression system for soluble biotinylated MHC-IIp proteins that makes use of steady lentiviral vector-transduced derivatives of HEK-293T cells. The expression design contains allele-specific peptide ligands tethered to the amino-terminus of the MHC-II β chain by way of a protease-cleavable linker. Following cleavage of the linker, HLA-DM is used to catalyze environment friendly peptide alternate, enabling high-throughput manufacturing of many distinct MHC-IIp complexes from a single manufacturing cell line.

Peptide alternate is monitored utilizing both of two label-free strategies, native isoelectric focusing gel electrophoresis or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of eluted peptides.

Together, these strategies produce MHC-IIp complexes which might be extremely homogeneous and that type the premise for excellent MHC-IIp multimer reagents. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Lentivirus manufacturing and expression line creation Support Protocol 1: Six-well assay for estimation of manufacturing cell line yield Support Protocol 2: Universal ELISA for quantifying proteins with fused leucine zippers and His-tags Basic Protocol 2: Cultures for manufacturing of Class II MHC proteins Basic

Protocol 3: Purification of Class II MHC proteins by anti-leucine zipper affinity chromatography Alternate Protocol 1: IMAC purification of His-tagged Class II MHC Support Protocol 3: Protein focus measurements and changes Support Protocol 4: Polishing purification by anion-exchange chromatography

Support Protocol 5: Estimating biotinylation share by streptavidin precipitation Basic Protocol 4: Peptide alternate Basic Protocol 5: Analysis of peptide alternate by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Alternate Protocol 2: Native isoelectric focusing to validate MHC-II peptide loading Basic Protocol 6: Multimerization Basic Protocol 7: Staining cells with Class II MHC tetramers.

Autonomous spheroid formation by tradition plate compartmentation

Scaffold-free 3D cell cultures (e.g. pellet cultures) are broadly used in medical science, together with cartilage regeneration. Their drawbacks are excessive time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid era, most tradition methods stay costly or require tedious procedures. The purpose of this research was to ascertain a system for useful resource environment friendly spheroid era.

This was achieved by compartmentation of cell tradition surfaces utilising laser engraving (grid plates). This compartmentation triggered autonomous spheroid formation by way of rolling-up of the cell monolayer in human adipose-derived stem cells (ASC/TERT1) and human articular chondrocytes (hAC)-ASC/TERT1 co-cultures, when cultivated on grid plates below chondrogenic circumstances. Plates with Three mm grid dimension yielded steady diameters (about 300 μm). ASC/TERT1 spheroids totally shaped inside

Three weeks whereas co-cultures took 1-2 weeks, forming considerably quicker with rising hAC ratio (p<0.05 and 0.01 for 1:1 and 1:Four ASC/TERT1:hAC ratio respectively). Co-cultures confirmed barely decrease spheroid diameter, attributable to earlier spheroid formation and incomplete monolayer formation. However, this was related to extra common matrix distribution in the co-tradition.

Both confirmed differentiation capability comparable to plain pellet tradition in (immune-)histochemistry and RT-qPCR. To assess usability for cartilage restore, spheroids have been embedded right into a hydrogel (fibrin), yielding cellular outgrowth and matrix deposition, which was particularly pronounced in co-cultures. The herein offered novel cell tradition system is just not solely a promising instrument for autonomous spheroid era with the potential of experimental and scientific software in tissue engineering but in addition for high-throughput evaluation for each pharmaceutical and therapeutic makes use of.

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Development of a novel β-1,6-glucan-specific detection system utilizing functionally-modified recombinant endo-β-1,6-glucanase

β-1,3D-Glucan is a ubiquitous glucose polymer produced by vegetation, micro organism, and most fungi. It has been used as a diagnostic instrument in sufferers with invasive mycoses by way of a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no methodology is presently obtainable for measuring β-1,6-glucan, one other main β-glucan construction of fungal polysaccharides.

Herein, we describe the event of a cheap and highly-sensitive and particular assay for β-1,6-glucan utilizing a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase exercise. The purified β-1,6-glucanase by-product certain to the β-1,6-glucan pustulan with a OkayD of 16.Four nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its capacity to detect cell wall β-1,6-glucan from each yeast and hyphal types of the opportunistic fungal pathogen Candida albicans, with none detectable binding to glucan missing the lengthy β-1,6-glucan department.

Mouse Complement Serum

32R-CM001 5 ml
EUR 435
Description: Frozen high activity mouse complement serum

Normal Mouse Serum

88-NM35 50 ml
EUR 516
Description: Normal Mouse Serum

Normal Mouse Serum

88R-1002 5 ml
EUR 203
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Mouse Serum Amyloid P Component (SAP) Reference serum

SAP12-RS 100 ul
EUR 324

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: ALB/398

APR07283G 0.05mg
EUR 396
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose

SPR-204A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose

SPR-204B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose

SPR-204C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein

SPR-206A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein

SPR-206B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein

SPR-206C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde

SPR-207A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde

SPR-207B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde

SPR-207C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal

SPR-208A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal

SPR-208B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal

SPR-208C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde

SPR-209A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde

SPR-209B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde

SPR-209C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Equine Serum

EQS001 500 ml
EUR 200

Chicken Serum

SER001 500 ml
EUR 265

Cow Serum

abx098251-100ml 100 ml
EUR 342

Fish Serum

abx098257-10ml 10 ml
EUR 439

Goat Serum

abx098258-500ml 500 ml
EUR 370

Goat Serum

abx098259-500ml 500 ml
EUR 370

Horse Serum

abx098260-100ml 100 ml
EUR 342

Porcine Serum

abx098264-1L 1 L
EUR 592

Rabbit Serum

abx098265-100ml 100 ml
EUR 272

Rabbit Serum

abx098266-500ml 500 ml
EUR 481

Rat Serum

abx098267-10ml 10 ml
EUR 272

Sheep Serum

abx098268-100ml 100 ml
EUR 342

Canine Serum

88R-D005S 50 ml
EUR 154
Description: Non Sterile Canine Serum

Rabbit Serum

1267-1
EUR 157

Mouse Serum albumin (Alb)

1-CSB-YP001561MO
  • EUR 586.00
  • EUR 299.00
  • EUR 2172.00
  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast

Mouse Serum albumin (Alb)

1-CSB-BP001561MO
  • EUR 840.00
  • EUR 332.00
  • EUR 2170.00
  • EUR 1135.00
  • EUR 1579.00
  • EUR 470.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus

Mouse Serum Albumin Protein

20-abx600021
  • EUR 926.00
  • EUR 314.00
  • EUR 3975.00
  • EUR 244.00
  • 100 mg
  • 10 mg
  • 1 g
  • 1 mg

Non-sterile mouse serum

MS05-0050 50 ml
EUR 231.4

Non-sterile mouse serum

MS05-0100 100 ml
EUR 257.4

Non-sterile mouse serum

MS05-0500 500 ml
EUR 440.7

Mouse Serum Albumin antibody

20R-AR005 20 mg
EUR 327
Description: Rabbit polyclonal Mouse Serum Albumin antibody

Mouse Serum albumin antibody

20R-AR041 2 ml
EUR 295
Description: Rabbit polyclonal Mouse Serum albumin antibody

Mouse Serum Albumin antibody

70R-AG001 1 mg
EUR 256
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody

Mouse Serum Albumin protein

30-1179 10 mg
EUR 236
Description: Purified native Mouse Albumin protein

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine

SPR-201A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine

SPR-201B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine

SPR-201C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine

SPR-202A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine

SPR-202B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine

SPR-202C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN

99-783-CM 1 L /pk
EUR 63
Description: Specialty Media; Islet products

WASH SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN

99-784-CM 1 L /pk
EUR 71
Description: Specialty Media; Islet products

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP 30 rxn
EUR 1001

Bovine Serum Albumin

HY-D0842 10g
EUR 187

Bovine Serum Albumin

GE1877-250G 250 g
EUR 285

Bovine Serum Albumin

GE1877-25G 25 g
EUR 70

Bovine Serum Albumin

GE1877-500G 500 g
EUR 500

Bovine Serum Albumin

GE1877-100G 100 g
EUR 158

Bovine Serum Albumin

GE1877-10G 10 g
EUR 54

Bovine Serum Albumin

GE1877-50G 50 g
EUR 102

Newborn Calf Serum

F0603-050 500ml
EUR 151

Doner Horse Serum

F0605-050 500ml
EUR 151

Human Serum AB

SC00B14 100 ml
EUR 362

Normal Goat Serum

9067 50 ml
EUR 100.75
Description: Normal Goat Serum

Serum Thymic Factor

5-01924 4 x 5mg Ask for price

Rabbit Serum (Sterile)

20-abx800002
  • EUR 1400.00
  • EUR 3293.00
  • EUR 829.00
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Sterile)

abx800002-1L 1 L
EUR 5437

Sheep Serum (Sterile)

20-abx800019
  • EUR 592.00
  • EUR 759.00
  • EUR 425.00
  • 100 ml
  • 500 ml
  • 50 ml

Sheep Serum (Sterile)

abx800019-1L 1 L
EUR 996

Goat Serum (Sterile)

20-abx800034
  • EUR 606.00
  • EUR 773.00
  • EUR 439.00
  • 100 ml
  • 500 ml
  • 50 ml

Goat Serum (Sterile)

abx800034-1L 1 L
EUR 926

Horse Serum (Sterile)

20-abx800049
  • EUR 829.00
  • EUR 1080.00
  • EUR 578.00
  • 100 ml
  • 500 ml
  • 50 ml

Horse Serum (Sterile)

abx800049-1L 1 L
EUR 1316

Donkey Serum (Sterile)

20-abx800064
  • EUR 1358.00
  • EUR 1803.00
  • EUR 746.00
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Serum (Sterile)

abx800064-1L 1 L
EUR 2221

Camel Serum (Sterile)

20-abx800079
  • EUR 1511.00
  • EUR 342.00
  • EUR 3460.00
  • EUR 913.00
  • 100 ml
  • 10 ml
  • 500 ml
  • 50 ml

Camel Serum (Sterile)

abx800079-1L 1 L
EUR 5924

Dog Serum (Sterile)

20-abx800109
  • EUR 1288.00
  • EUR 2973.00
  • EUR 759.00
  • 100 ml
  • 500 ml
  • 50 ml

Dog Serum (Sterile)

abx800109-1L 1 L
EUR 4935

Chicken Serum (Sterile)

20-abx800124
  • EUR 746.00
  • EUR 1609.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml

Chicken Serum (Sterile)

abx800124-1L 1 L
EUR 2583

Normal Bovine Serum

abx098263-100ml 100 ml
EUR 258

Albumin Human Serum

abx082261-250mg 250 mg
EUR 203

Bovine Serum Albumin

7-04585 10gr Ask for price

Bovine Serum Albumin

7-04586 50gr Ask for price

Bovine Serum Albumin

7-04587 100gr Ask for price

Human Serum Albumin

7-05389 100mg Ask for price

Human Serum Albumin

7-05390 500mg Ask for price

Human Serum Albumin

7-05391 5gram Ask for price

Serum Albumin Protein

20-abx263082
  • EUR 230.00
  • EUR 328.00
  • 100 mg
  • 500 mg

Serum Albumin Protein

abx263082-5gr 5 gr
EUR 1066

Serum Albumin Antibody

48145-100ul 100ul
EUR 333

Serum Albumin Antibody

48145-50ul 50ul
EUR 239

Goat Serum antibody

20-S1121G000-V0 10 ml
EUR 133
Description: Guinea pig polyclonal Goat Serum antibody

Goat Serum antibody

20-S1121GN00-D0 10 ml
EUR 133
Description: Guinea pig polyclonal Goat Serum antibody

Human Serum Albumin

30R-2429 10 mg
EUR 597
Description: Purified native Human Serum Albumin

Rat Serum Albumin

30R-3297 10 mg
EUR 187
Description: Purified native Rat Serum Albumin

Pigeon Serum Albumin

30R-3298 10 mg
EUR 327
Description: Purified native Pigeon Serum Albumin

Chicken Serum Albumin

30R-3299 10 mg
EUR 187
Description: Purified native Chicken Serum Albumin

Horse Serum Albumin

30R-3300 10 mg
EUR 257
Description: Purified native Horse Serum Albumin

Sheep Serum Albumin

30R-3301 10 mg
EUR 257
Description: Purified native Sheep Serum Albumin

Human Serum Albumin

30R-3302 10 mg
EUR 349
Description: Purified native Human Serum Albumin

Rabbit Serum Albumin

30R-3303 10 mg
EUR 187
Description: Purified native Rabbit Serum Albumin

Goat Serum Albumin

30R-3305 10 mg
EUR 257
Description: Purified native Goat Serum Albumin

Porcine Serum Albumin

30R-3307 10 mg
EUR 187
Description: Purified native Porcine serum albumin

Canine Serum Albumin

30R-3308 10 mg
EUR 327
Description: Purified native Canine Serum Albumin

Hamster Complement Serum

32R-CH001 5 ml
EUR 424
Description: Purified normal Hamster Complement isolated from blood

Rabbit Complement Serum

32R-CR001 25 ml
EUR 241
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Rat Complement Serum

32R-CR005 5 ml
EUR 435
Description: Purifed Rat Complement isolated from blood of normal rats

Normal Goat Serum

88-B9999G000-S0 10 ml
EUR 133
Description: Control Normal Goat serum

Normal Canine Serum

88-NC25 100 ml
EUR 606
Description: Normal Canine Serum

Normal Horse Serum

88-NE21 100 ml
EUR 30
Description: Control Normal Horse Serum

Normal Goat Serum

88-NG22 500 ml
EUR 30
Description: Control Normal Goat Serum

Normal Goat Serum

88-NG22S 500 ml
EUR 191
Description: Control Normal Goat Serum

Normal Pig Serum

88-NP 500 ml
EUR 243
Description: Normal Pig Serum collected from pigs in the USA

Normal Rabbit Serum

88-NR50 100 ml
EUR 150
Description: Normal Rabbit Serum

Normal Rat Serum

88-NR51 50 ml
EUR 599
Description: Normal Rat Serum

Normal Sheep Serum

88-NS55 100 ml
EUR 126
Description: Normal Sheep Serum

Normal Rabbit Serum

88R-1004 10 ml
EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Goat Serum

88R-1006 10 ml
EUR 182
Description: Normal Goat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Sheep Serum

88R-1008 10 ml
EUR 182
Description: Normal Sheep Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat Serum

88R-1010 5 ml
EUR 202
Description: Normal Rat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Chicken Serum

88R-1014 5 ml
EUR 176
Description: Normal Chicken Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Horse Serum

88R-1020 10 ml
EUR 182
Description: Normal Horse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum

88R-1022 10 ml
EUR 182
Description: Normal Donkey Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Pig Serum

88R-1023 2 ml
EUR 116
Description: Normal Pig (Swine) Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Cat Serum

88R-1026 10 ml
EUR 250
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Dog Serum

88R-1028 10 ml
EUR 251
Description: Normal Dog Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Equine Serum Albumin

88R-1048 100 gram
EUR 2509
Description: Equine Serum Albumin standard grade powder.

Normal Bovine Serum

88R-B001 100 ml
EUR 165
Description: Control Normal Bovine Serum

Normal Donkey Serum

88R-D001 100 ml
EUR 179
Description: Control Normal Donkey Serum

Normal Donkey Serum

88R-D003 10 ml
EUR 149
Description: Control Normal Donkey Serum

Normal Goat Serum

88R-NG001 50 ml
EUR 117
Description: Normal Goat Serum for use as a control or blocking reagent

Normal Pig Serum

88R-P004 1 liter
EUR 673
Description: Control Normal Pig Serum

Normal Sheep Serum

88R-S002 100 ml
EUR 165
Description: Control Normal Sheep Serum

Normal Rabbit Serum

89R-RM001 20 mg
EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RM002 5 ml
EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RR001 20 mg
EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum

89R-RR002 5 ml
EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Defibrinated Human Serum

90-1010 10 ml
EUR 127
Description: Defibrinated Human Serum for use in Controls

Normal Human Serum

90R-1002 10 ml
EUR 254
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Steroid Free Serum

90R-1007 50 mL
EUR 392
Description: Steroid Free Human Serum

ORANGE SERUM AGAR

O15-101-10kg 10 kg
EUR 1729

ORANGE SERUM AGAR

O15-101-2Kg 2 Kg
EUR 415

ORANGE SERUM AGAR

O15-101-500g 500 g
EUR 149

ORANGE SERUM BROTH

O15-106-10kg 10 kg
EUR 1033

ORANGE SERUM BROTH

O15-106-2Kg 2 Kg
EUR 264

ORANGE SERUM BROTH

O15-106-500g 500 g
EUR 108

Human Serum Albumin

4016-10MG
EUR 495

Human Serum Albumin

4016-1MG
EUR 180

Hemopexin, Rat Serum

7545-100
EUR 283

Mouse Serum response factor (Srf)

1-CSB-EP881354MO
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum response factor(Srf) expressed in E.coli

Mouse Serum Albumin Polyclonal Antibody

A57545 100 µg
EUR 570.55
Description: fast delivery possible

Mouse serum albumin lyophilized powder

MSA62-0050 50mg
EUR 370.5

Mouse serum albumin lyophilized powder

MSA62-0100 100mg
EUR 509.6

Mouse serum albumin lyophilized powder

MSA62-0500 500mg
EUR 1311.7

Mouse serum albumin lyophilized powder

MSA62-1000 1gm Ask for price

Mouse control serum, Balb/c

NMOS-5BS 5 ml
EUR 164

Mouse control serum, CD-1

NMOS-CD1-1 1 ml
EUR 103

Mouse control serum, CD-1

NMOS-CD1-5 5 ml
EUR 164

Resistin (mouse) Serum ELISA Kit

K4920-100
EUR 958

We developed a sandwich ELISA-like assay with a low restrict of quantification for pustulan (1.5 pg/ml), and we efficiently employed this assay in the quantification of furthercellular β-1,6-glucan launched by >250 patient-derived strains of completely different Candida species (together with Candida auris) in tradition supernatant in vitro. We additionally used this assay to measure β-1,6-glucan in vivo in the serum and in a number of organs in a mouse mannequin of systemic candidiasis. Our work describes a dependable methodology for β-1,6-glucan detection, which can show helpful for the prognosis of invasive fungal infections.