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Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Posted by Sebastian Hernandez on in Antibodies, Assay Kits, Biology Cells, Biosafety Studies of a Clinically Applicable Lentiviral Vector for the Gene Therapy of Artemis-SCID., Clia Kits, Culture Cells, DNA, DNA Templates, DNA Testing, Effect of Medication Co-payment Vouchers on P2Y12 Inhibitor Use and Major Adverse Cardiovascular Events Among Patients With Myocardial Infarction: The ARTEMIS Randomized Clinical Trial., Elisa Kits, Equipments, Exosomes, Gels, Isotypes, My Blog, NATtrol, Panel, Particles, Pcr Kits, Peptides, Reagents, Ria Kits, RNA, Vector & Virus
Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Class II main histocompatibility advanced peptide (MHC-IIp) multimers are exactly engineered reagents used to detect T cells particular for antigens from pathogens, tumors, and self-proteins. While the associated Class I MHC/peptide (MHC-Ip) multimers are often produced from subunits expressed in E. coli, most Class II MHC alleles can’t be produced in micro organism, and this has contributed to the notion that MHC-IIp reagents are tougher to supply.

Herein, we current a sturdy constitutive expression system for soluble biotinylated MHC-IIp proteins that makes use of steady lentiviral vector-transduced derivatives of HEK-293T cells. The expression design contains allele-specific peptide ligands tethered to the amino-terminus of the MHC-II β chain by way of a protease-cleavable linker. Following cleavage of the linker, HLA-DM is used to catalyze environment friendly peptide alternate, enabling high-throughput manufacturing of many distinct MHC-IIp complexes from a single manufacturing cell line.

Peptide alternate is monitored utilizing both of two label-free strategies, native isoelectric focusing gel electrophoresis or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of eluted peptides.

Together, these strategies produce MHC-IIp complexes which might be extremely homogeneous and that type the premise for excellent MHC-IIp multimer reagents. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Lentivirus manufacturing and expression line creation Support Protocol 1: Six-well assay for estimation of manufacturing cell line yield Support Protocol 2: Universal ELISA for quantifying proteins with fused leucine zippers and His-tags Basic Protocol 2: Cultures for manufacturing of Class II MHC proteins Basic

Protocol 3: Purification of Class II MHC proteins by anti-leucine zipper affinity chromatography Alternate Protocol 1: IMAC purification of His-tagged Class II MHC Support Protocol 3: Protein focus measurements and changes Support Protocol 4: Polishing purification by anion-exchange chromatography

Support Protocol 5: Estimating biotinylation share by streptavidin precipitation Basic Protocol 4: Peptide alternate Basic Protocol 5: Analysis of peptide alternate by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Alternate Protocol 2: Native isoelectric focusing to validate MHC-II peptide loading Basic Protocol 6: Multimerization Basic Protocol 7: Staining cells with Class II MHC tetramers.

Autonomous spheroid formation by tradition plate compartmentation

Scaffold-free 3D cell cultures (e.g. pellet cultures) are broadly used in medical science, together with cartilage regeneration. Their drawbacks are excessive time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid era, most tradition methods stay costly or require tedious procedures. The purpose of this research was to ascertain a system for useful resource environment friendly spheroid era.

This was achieved by compartmentation of cell tradition surfaces utilising laser engraving (grid plates). This compartmentation triggered autonomous spheroid formation by way of rolling-up of the cell monolayer in human adipose-derived stem cells (ASC/TERT1) and human articular chondrocytes (hAC)-ASC/TERT1 co-cultures, when cultivated on grid plates below chondrogenic circumstances. Plates with Three mm grid dimension yielded steady diameters (about 300 μm). ASC/TERT1 spheroids totally shaped inside

Three weeks whereas co-cultures took 1-2 weeks, forming considerably quicker with rising hAC ratio (p<0.05 and 0.01 for 1:1 and 1:Four ASC/TERT1:hAC ratio respectively). Co-cultures confirmed barely decrease spheroid diameter, attributable to earlier spheroid formation and incomplete monolayer formation. However, this was related to extra common matrix distribution in the co-tradition.

Both confirmed differentiation capability comparable to plain pellet tradition in (immune-)histochemistry and RT-qPCR. To assess usability for cartilage restore, spheroids have been embedded right into a hydrogel (fibrin), yielding cellular outgrowth and matrix deposition, which was particularly pronounced in co-cultures. The herein offered novel cell tradition system is just not solely a promising instrument for autonomous spheroid era with the potential of experimental and scientific software in tissue engineering but in addition for high-throughput evaluation for each pharmaceutical and therapeutic makes use of.

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Development of a novel β-1,6-glucan-specific detection system utilizing functionally-modified recombinant endo-β-1,6-glucanase

β-1,3D-Glucan is a ubiquitous glucose polymer produced by vegetation, micro organism, and most fungi. It has been used as a diagnostic instrument in sufferers with invasive mycoses by way of a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no methodology is presently obtainable for measuring β-1,6-glucan, one other main β-glucan construction of fungal polysaccharides.

Herein, we describe the event of a cheap and highly-sensitive and particular assay for β-1,6-glucan utilizing a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase exercise. The purified β-1,6-glucanase by-product certain to the β-1,6-glucan pustulan with a OkayD of 16.Four nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its capacity to detect cell wall β-1,6-glucan from each yeast and hyphal types of the opportunistic fungal pathogen Candida albicans, with none detectable binding to glucan missing the lengthy β-1,6-glucan department.

Mouse Complement Serum
32R-CM001 5 ml
EUR 435
Description: Frozen high activity mouse complement serum

Normal Mouse Serum
88-NM35 50 ml
EUR 516
Description: Normal Mouse Serum

Normal Mouse Serum
88R-1002 5 ml
EUR 203
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Mouse Serum Amyloid P Component (SAP) Reference serum
SAP12-RS 100 ul
EUR 324

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: ALB/398
APR07283G 0.05mg
EUR 396
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

Glycated Bovine Serum Albumin (BSA) with ?-Galactose
SPR-203A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose
SPR-203B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose
SPR-203C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose
SPR-204A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose
SPR-204B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose
SPR-204C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein
SPR-206A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein
SPR-206B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein
SPR-206C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde
SPR-207A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde
SPR-207B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde
SPR-207C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal
SPR-208A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal
SPR-208B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal
SPR-208C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde
SPR-209A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde
SPR-209B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde
SPR-209C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Equine Serum
EQS001 500 ml
EUR 200

Chicken Serum
SER001 500 ml
EUR 265

Cow Serum
abx098251-100ml 100 ml
EUR 342

Fish Serum
abx098257-10ml 10 ml
EUR 439

Goat Serum
abx098258-500ml 500 ml
EUR 370

Goat Serum
abx098259-500ml 500 ml
EUR 370

Horse Serum
abx098260-100ml 100 ml
EUR 342

Porcine Serum
abx098264-1L 1 L
EUR 592

Rabbit Serum
abx098265-100ml 100 ml
EUR 272

Rabbit Serum
abx098266-500ml 500 ml
EUR 481

Rat Serum
abx098267-10ml 10 ml
EUR 272

Sheep Serum
abx098268-100ml 100 ml
EUR 342

Canine Serum
88R-D005S 50 ml
EUR 154
Description: Non Sterile Canine Serum

Rabbit Serum
1267-1
EUR 157

Mouse Serum albumin (Alb)
1-CSB-YP001561MO
  • EUR 586.00
  • EUR 299.00
  • EUR 2172.00
  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast

Mouse Serum albumin (Alb)
1-CSB-BP001561MO
  • EUR 840.00
  • EUR 332.00
  • EUR 2170.00
  • EUR 1135.00
  • EUR 1579.00
  • EUR 470.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus

Mouse Serum Albumin Protein
20-abx600021
  • EUR 926.00
  • EUR 314.00
  • EUR 3975.00
  • EUR 244.00
  • 100 mg
  • 10 mg
  • 1 g
  • 1 mg

Non-sterile mouse serum
MS05-0050 50 ml
EUR 231.4

Non-sterile mouse serum
MS05-0100 100 ml
EUR 257.4

Non-sterile mouse serum
MS05-0500 500 ml
EUR 440.7

Mouse Serum Albumin antibody
20R-AR005 20 mg
EUR 327
Description: Rabbit polyclonal Mouse Serum Albumin antibody

Mouse Serum albumin antibody
20R-AR041 2 ml
EUR 295
Description: Rabbit polyclonal Mouse Serum albumin antibody

Mouse Serum Albumin antibody
70R-AG001 1 mg
EUR 256
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody

Mouse Serum Albumin protein
30-1179 10 mg
EUR 236
Description: Purified native Mouse Albumin protein

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
SPR-201A 0.05 mg
EUR 256
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
SPR-201B 2x0.05 mg
EUR 370
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
SPR-201C 4x0.05 mg
EUR 523
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine
SPR-202A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine
SPR-202B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine
SPR-202C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-783-CM 1 L /pk
EUR 63
Description: Specialty Media; Islet products

WASH SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-784-CM 1 L /pk
EUR 71
Description: Specialty Media; Islet products

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP 30 rxn
EUR 1001

Bovine Serum Albumin
HY-D0842 10g
EUR 187

Bovine Serum Albumin
GE1877-250G 250 g
EUR 285

Bovine Serum Albumin
GE1877-25G 25 g
EUR 70

Bovine Serum Albumin
GE1877-500G 500 g
EUR 500

Bovine Serum Albumin
GE1877-100G 100 g
EUR 158

Bovine Serum Albumin
GE1877-10G 10 g
EUR 54

Bovine Serum Albumin
GE1877-50G 50 g
EUR 102

Newborn Calf Serum
F0603-050 500ml
EUR 151

Doner Horse Serum
F0605-050 500ml
EUR 151

Human Serum AB
SC00B14 100 ml
EUR 362

Normal Goat Serum
9067 50 ml
EUR 100.75
Description: Normal Goat Serum

Serum Thymic Factor
5-01924 4 x 5mg Ask for price

Rabbit Serum (Sterile)
20-abx800002
  • EUR 1400.00
  • EUR 3293.00
  • EUR 829.00
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Sterile)
abx800002-1L 1 L
EUR 5437

Sheep Serum (Sterile)
20-abx800019
  • EUR 592.00
  • EUR 759.00
  • EUR 425.00
  • 100 ml
  • 500 ml
  • 50 ml

Sheep Serum (Sterile)
abx800019-1L 1 L
EUR 996

Goat Serum (Sterile)
20-abx800034
  • EUR 606.00
  • EUR 773.00
  • EUR 439.00
  • 100 ml
  • 500 ml
  • 50 ml

Goat Serum (Sterile)
abx800034-1L 1 L
EUR 926

Horse Serum (Sterile)
20-abx800049
  • EUR 829.00
  • EUR 1080.00
  • EUR 578.00
  • 100 ml
  • 500 ml
  • 50 ml

Horse Serum (Sterile)
abx800049-1L 1 L
EUR 1316

Donkey Serum (Sterile)
20-abx800064
  • EUR 1358.00
  • EUR 1803.00
  • EUR 746.00
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Serum (Sterile)
abx800064-1L 1 L
EUR 2221

Camel Serum (Sterile)
20-abx800079
  • EUR 1511.00
  • EUR 342.00
  • EUR 3460.00
  • EUR 913.00
  • 100 ml
  • 10 ml
  • 500 ml
  • 50 ml

Camel Serum (Sterile)
abx800079-1L 1 L
EUR 5924

Dog Serum (Sterile)
20-abx800109
  • EUR 1288.00
  • EUR 2973.00
  • EUR 759.00
  • 100 ml
  • 500 ml
  • 50 ml

Dog Serum (Sterile)
abx800109-1L 1 L
EUR 4935

Chicken Serum (Sterile)
20-abx800124
  • EUR 746.00
  • EUR 1609.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml

Chicken Serum (Sterile)
abx800124-1L 1 L
EUR 2583

Normal Bovine Serum
abx098263-100ml 100 ml
EUR 258

Albumin Human Serum
abx082261-250mg 250 mg
EUR 203

Bovine Serum Albumin
7-04585 10gr Ask for price

Bovine Serum Albumin
7-04586 50gr Ask for price

Bovine Serum Albumin
7-04587 100gr Ask for price

Human Serum Albumin
7-05389 100mg Ask for price

Human Serum Albumin
7-05390 500mg Ask for price

Human Serum Albumin
7-05391 5gram Ask for price

Serum Albumin Protein
20-abx263082
  • EUR 230.00
  • EUR 328.00
  • 100 mg
  • 500 mg

Serum Albumin Protein
abx263082-5gr 5 gr
EUR 1066

Serum Albumin Antibody
48145-100ul 100ul
EUR 333

Serum Albumin Antibody
48145-50ul 50ul
EUR 239

Goat Serum antibody
20-S1121G000-V0 10 ml
EUR 133
Description: Guinea pig polyclonal Goat Serum antibody

Goat Serum antibody
20-S1121GN00-D0 10 ml
EUR 133
Description: Guinea pig polyclonal Goat Serum antibody

Human Serum Albumin
30R-2429 10 mg
EUR 597
Description: Purified native Human Serum Albumin

Rat Serum Albumin
30R-3297 10 mg
EUR 187
Description: Purified native Rat Serum Albumin

Pigeon Serum Albumin
30R-3298 10 mg
EUR 327
Description: Purified native Pigeon Serum Albumin

Chicken Serum Albumin
30R-3299 10 mg
EUR 187
Description: Purified native Chicken Serum Albumin

Horse Serum Albumin
30R-3300 10 mg
EUR 257
Description: Purified native Horse Serum Albumin

Sheep Serum Albumin
30R-3301 10 mg
EUR 257
Description: Purified native Sheep Serum Albumin

Human Serum Albumin
30R-3302 10 mg
EUR 349
Description: Purified native Human Serum Albumin

Rabbit Serum Albumin
30R-3303 10 mg
EUR 187
Description: Purified native Rabbit Serum Albumin

Goat Serum Albumin
30R-3305 10 mg
EUR 257
Description: Purified native Goat Serum Albumin

Porcine Serum Albumin
30R-3307 10 mg
EUR 187
Description: Purified native Porcine serum albumin

Canine Serum Albumin
30R-3308 10 mg
EUR 327
Description: Purified native Canine Serum Albumin

Hamster Complement Serum
32R-CH001 5 ml
EUR 424
Description: Purified normal Hamster Complement isolated from blood

Rabbit Complement Serum
32R-CR001 25 ml
EUR 241
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Rat Complement Serum
32R-CR005 5 ml
EUR 435
Description: Purifed Rat Complement isolated from blood of normal rats

Normal Goat Serum
88-B9999G000-S0 10 ml
EUR 133
Description: Control Normal Goat serum

Normal Canine Serum
88-NC25 100 ml
EUR 606
Description: Normal Canine Serum

Normal Horse Serum
88-NE21 100 ml
EUR 30
Description: Control Normal Horse Serum

Normal Goat Serum
88-NG22 500 ml
EUR 30
Description: Control Normal Goat Serum

Normal Goat Serum
88-NG22S 500 ml
EUR 191
Description: Control Normal Goat Serum

Normal Pig Serum
88-NP 500 ml
EUR 243
Description: Normal Pig Serum collected from pigs in the USA

Normal Rabbit Serum
88-NR50 100 ml
EUR 150
Description: Normal Rabbit Serum

Normal Rat Serum
88-NR51 50 ml
EUR 599
Description: Normal Rat Serum

Normal Sheep Serum
88-NS55 100 ml
EUR 126
Description: Normal Sheep Serum

Normal Rabbit Serum
88R-1004 10 ml
EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Goat Serum
88R-1006 10 ml
EUR 182
Description: Normal Goat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Sheep Serum
88R-1008 10 ml
EUR 182
Description: Normal Sheep Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat Serum
88R-1010 5 ml
EUR 202
Description: Normal Rat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Chicken Serum
88R-1014 5 ml
EUR 176
Description: Normal Chicken Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Horse Serum
88R-1020 10 ml
EUR 182
Description: Normal Horse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum
88R-1022 10 ml
EUR 182
Description: Normal Donkey Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Pig Serum
88R-1023 2 ml
EUR 116
Description: Normal Pig (Swine) Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Cat Serum
88R-1026 10 ml
EUR 250
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Dog Serum
88R-1028 10 ml
EUR 251
Description: Normal Dog Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Equine Serum Albumin
88R-1048 100 gram
EUR 2509
Description: Equine Serum Albumin standard grade powder.

Normal Bovine Serum
88R-B001 100 ml
EUR 165
Description: Control Normal Bovine Serum

Normal Donkey Serum
88R-D001 100 ml
EUR 179
Description: Control Normal Donkey Serum

Normal Donkey Serum
88R-D003 10 ml
EUR 149
Description: Control Normal Donkey Serum

Normal Goat Serum
88R-NG001 50 ml
EUR 117
Description: Normal Goat Serum for use as a control or blocking reagent

Normal Pig Serum
88R-P004 1 liter
EUR 673
Description: Control Normal Pig Serum

Normal Sheep Serum
88R-S002 100 ml
EUR 165
Description: Control Normal Sheep Serum

Normal Rabbit Serum
89R-RM001 20 mg
EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum
89R-RM002 5 ml
EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum
89R-RR001 20 mg
EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum
89R-RR002 5 ml
EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Defibrinated Human Serum
90-1010 10 ml
EUR 127
Description: Defibrinated Human Serum for use in Controls

Normal Human Serum
90R-1002 10 ml
EUR 254
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Steroid Free Serum
90R-1007 50 mL
EUR 392
Description: Steroid Free Human Serum

ORANGE SERUM AGAR
O15-101-10kg 10 kg
EUR 1729

ORANGE SERUM AGAR
O15-101-2Kg 2 Kg
EUR 415

ORANGE SERUM AGAR
O15-101-500g 500 g
EUR 149

ORANGE SERUM BROTH
O15-106-10kg 10 kg
EUR 1033

ORANGE SERUM BROTH
O15-106-2Kg 2 Kg
EUR 264

ORANGE SERUM BROTH
O15-106-500g 500 g
EUR 108

Human Serum Albumin
4016-10MG
EUR 495

Human Serum Albumin
4016-1MG
EUR 180

Hemopexin, Rat Serum
7545-100
EUR 283

Mouse Serum response factor (Srf)
1-CSB-EP881354MO
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum response factor(Srf) expressed in E.coli

Mouse Serum Albumin Polyclonal Antibody
A57545 100 µg
EUR 570.55
Description: fast delivery possible

Mouse serum albumin lyophilized powder
MSA62-0050 50mg
EUR 370.5

Mouse serum albumin lyophilized powder
MSA62-0100 100mg
EUR 509.6

Mouse serum albumin lyophilized powder
MSA62-0500 500mg
EUR 1311.7

Mouse serum albumin lyophilized powder
MSA62-1000 1gm Ask for price

Mouse control serum, Balb/c
NMOS-5BS 5 ml
EUR 164

Mouse control serum, CD-1
NMOS-CD1-1 1 ml
EUR 103

Mouse control serum, CD-1
NMOS-CD1-5 5 ml
EUR 164

Resistin (mouse) Serum ELISA Kit
K4920-100
EUR 958

We developed a sandwich ELISA-like assay with a low restrict of quantification for pustulan (1.5 pg/ml), and we efficiently employed this assay in the quantification of furthercellular β-1,6-glucan launched by >250 patient-derived strains of completely different Candida species (together with Candida auris) in tradition supernatant in vitro. We additionally used this assay to measure β-1,6-glucan in vivo in the serum and in a number of organs in a mouse mannequin of systemic candidiasis. Our work describes a dependable methodology for β-1,6-glucan detection, which can show helpful for the prognosis of invasive fungal infections.

Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line

Posted by Sebastian Hernandez on in Biology Cells, Biosafety Studies of a Clinically Applicable Lentiviral Vector for the Gene Therapy of Artemis-SCID., My Blog, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Ria Kits, RNA, Vector & Virus
A difficult facet with the use of protein hydrolysates in business manufacturing processes of recombinant therapeutic proteins is their impacts on the protein production resulting from a lack of understanding of batch-to-batch variability. Soy hydrolysates variability and its influence on fed-batch production of a recombinant monoclonal antibody (mAb) expressed in Sp2/zero cells have been studied utilizing 37 batches from the identical vendor.
The batch-to-batch variability of soy hydrolysates impacted cell development, titer and product high quality. Physico-chemical characterization of batches confirmed that soy hydrolysates are primarily a supply of amino acids and peptides containing decrease quantities of different parts corresponding to carbohydrates and chemical parts in cell tradition media. Soy hydrolysates composition of completely different batches was constant apart from hint parts. Statistical analyses recognized iron as a potential marker of a poor course of efficiency.
To confirm this correlation, two varieties of iron, ferric ammonium citrate and ferrous sulfate, have been added to a batch of soy hydrolysates related to a low stage of iron throughout cell tradition. Both varieties of iron decreased considerably cell development, mAb titer and elevated stage of the acidic cost variants of the mAb. Consequently, hint factor composition of soy hydrolysates or of all incoming uncooked supplies may result in important impacts on course of efficiency and product high quality and due to this fact must be tightly managed.

Mitochondrial dysfunction and oxidative stress in neurodegenerative illnesses.

Many strains of proof counsel that mitochondria have a central function in ageing-related neurodegenerative illnesses. Mitochondria are vital regulators of cell loss of life, a key characteristic of neurodegeneration. Mutations in mitochondrial DNA and oxidative stress each contribute to ageing, which is the best threat issue for neurodegenerative illnesses. In all main examples of these illnesses there may be sturdy proof that mitochondrial dysfunction happens early and acts causally in illness pathogenesis.
Moreover, a formidable quantity of disease-specific proteins work together with mitochondria. Thus, therapies concentrating on primary mitochondrial processes, corresponding to vitality metabolism or free-radical era, or particular interactions of disease-related proteins with mitochondria, maintain nice promise.

Glioblastoma microvesicles transport RNA and proteins that promote tumour development and present diagnostic biomarkers.

Glioblastoma tumour cells launch microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by regular host cells, corresponding to mind microvascular endothelial cells. By incorporating an mRNA for a reporter protein into these microvesicles, we display that messages delivered by microvesicles are translated by recipient cells. These microvesicles are additionally enriched in angiogenic proteins and stimulate tubule formation by endothelial cells. Tumour-derived microvesicles due to this fact function a means of delivering genetic data and proteins to recipient cells within the tumour surroundings.
Glioblastoma microvesicles additionally stimulated proliferation of a human glioma cell line, indicating a self-promoting facet. Messenger RNA mutant/variants and miRNAs attribute of gliomas could possibly be detected in serum microvesicles of glioblastoma sufferers. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma sufferers. Thus, tumour-derived microvesicles could present diagnostic data and help in therapeutic choices for most cancers sufferers by way of a blood take a look at.

Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic standards.

Endothelial cells have been remoted from freshly obtained human umbilical cords by collagenase digestion of the inside of the umbilical vein. The cells have been grown in tissue tradition as a homogeneous inhabitants for durations as much as 5 mo and some strains have been subcultured for 10 serial passages. During the logarithmic section of cell development, cell-doubling time was 92 h. Light, section distinction, and scanning electron microscopy demonstrated that cultured human endothelial cells grew as monolayers of intently opposed, polygonal massive cells whereas each cultured human fibroblasts and human easy muscle cells grew as overlapping layers of parallel arrays of slender, spindle-shaped cells.
By transmission electron microscopy, cultured endothelial cells have been seen to comprise cytoplasmic inclusions (Weibel-Palade our bodies) attribute of in situ endothelial cells. These inclusions have been additionally present in endothelial cells lining umbilical veins however weren’t seen in easy muscle cells or fibroblasts in tradition or in situ. Cultured endothelial cells contained ample portions of easy muscle actomyosin. Cultured endothelial cells additionally contained ABH antigens applicable to the tissue donor’s blood kind; these antigens weren’t detectable on cultured easy muscle cells or fibroblasts. These research display that it’s attainable to tradition morphologically and immunologically identifiable human endothelial cells for durations as much as 5 mo.

Generation of germline-competent induced pluripotent stem cells.

We have beforehand proven that pluripotent stem cells will be induced from mouse fibroblasts by retroviral introduction of Oct3/4 (additionally referred to as Pou5f1), Sox2, c-Myc and Klf4, and subsequent choice for Fbx15 (additionally referred to as Fbxo15) expression. These induced pluripotent stem (iPS) cells (hereafter referred to as Fbx15 iPS cells) are much like embryonic stem (ES) cells in morphology, proliferation and teratoma formation; nonetheless, they’re completely different almost about gene expression and DNA methylation patterns, and fail to supply grownup chimaeras.
Here we present that choice for Nanog expression leads to germline-competent iPS cells with elevated ES-cell-like gene expression and DNA methylation patterns in contrast with Fbx15 iPS cells. The 4 transgenes (Oct3/4, Sox2, c-myc and Klf4) have been strongly silenced in Nanog iPS cells. We obtained grownup chimaeras from seven Nanog iPS cell clones, with one clone being transmitted by way of the germ line to the subsequent era.
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Approximately 20% of the offspring developed tumours attributable to reactivation of the c-myc transgene. Thus, iPS cells competent for germline chimaeras will be obtained from fibroblasts, however retroviral introduction of c-Myc ought to be averted for medical utility.