Month: april 2021

[Effects and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum]

Posted by Sebastian Hernandez on in Antibodies, Assay Kits, Biology Cells, Biosafety Studies of a Clinically Applicable Lentiviral Vector for the Gene Therapy of Artemis-SCID., CD3ε+ Cells in Pigs With Severe Combined Immunodeficiency Due to Defects in ARTEMIS., cDNA, Clia Kits, Culture Cells, DNA, DNA Templates, DNA Testing, Effect of Medication Co-payment Vouchers on P2Y12 Inhibitor Use and Major Adverse Cardiovascular Events Among Patients With Myocardial Infarction: The ARTEMIS Randomized Clinical Trial., Elisa Kits, Equipments, Exosomes, Gels, Isotypes, My Blog, NATtrol, Particles, PCR, Pcr Kits, Peptides, Reagents, Ria Kits, RNA, Vector & Virus
[Effects and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum]

To examine the impact and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum. The experimental technique was used. Ten eight-month-old male Sprague-Dawley (SD) rats have been taken to arrange regular rat serum (hereinafter known as regular serum), 30 eight-month-old male SD rats have been taken to arrange burn rat serum (hereinafter known as burn serum) 6 hours after full-thickness burn, and the third passage of epidermal cells collected from 10 one-day-old SD rats have been used for the experiments.

The cells have been divided into regular serum group handled with regular serum and burn serum group handled with burn serum. Cell counting package eight technique was used to detect cell survival fee after 1, 2, 4, 6, and eight h of tradition, respectively, to display the following intervention time of burn serum. The cells have been divided into burn serum management group handled solely with burn serum and 50, 100, 150, 200, 250 μmol/L sodium hydrosulfide teams handled with burn serum+sodium hydrosulfide at corresponding last molarity.

After 30 min of tradition following the burn serum intervention, the cell survival fee was measured as above to display the following intervention focus of sodium hydrosulfide. The cells have been divided into burn serum management group handled with burn serum solely and sodium hydrosulfide solely group, glibenclamide solely group, and sodium hydrosulfide+glibenclamide group handled with burn serum+corresponding reagents. After 5, 10, 15 min of tradition following the burn serum intervention, the cell survival fee was measured as above to display the following intervention time of glibenclamide. The cells have been divided into burn serum management group handled with burn serum and sodium hydrosulfide solely group, glibenclamide solely group, and sodium hydrosulfide+glibenclamide group handled with burn serum+corresponding reagents.

After finishing corresponding tradition time of every reagent, the mitochondria have been extracted to detect cytochrome c oxidase (CCO) exercise utilizing a spectrophotometer, and the protein expression stage of adenosine triphosphate (ATP)-sensitive potassium channel was detected by Western blotting. Except for the quantity of samples for ATP-sensitive potassium channel protein detection, which was 3, the quantity of samples for the opposite indicators was 10. Data have been statistically analyzed with evaluation of variance for factorial design, one-way evaluation of variance, least important distinction (LSD)-t take a look at, LSD take a look at, and Bonferroni correction.

Compared with that of regular serum group, the survival fee of cells was considerably decreased in burn serum group after solely 4 and 6 h of tradition (t=4.02, 6.42, P<0.05). There have been statistically important variations among the many time factors inside regular serum group and burn serum group in survival fee of cells (F=19.74, 4.48, P<0.05 or P<0.01). Culture of Four h was chosen as the following intervention time of burn serum. After 30 min of tradition following the burn serum intervention, in contrast with that of burn serum management group, solely the 150, 200, 250 μmol/L sodium hydrosulfide teams had a considerably larger survival fee of cells (P<0.05), thus 150 μmol/L was chosen as the following intervention focus of sodium hydrosulfide.

Compared with that of burn serum management group, the survival fee of cells decreased considerably in glibenclamide solely group after 5 and 15 min of tradition following the burn serum intervention (P<0.05) and elevated considerably in glibenclamide solely group after 10 min of tradition following the burn serum intervention and sodium hydrosulfide solely group at every time level (P<0.05 or P<0.01). The survival fee of cells in hydrosulfide+glibenclamide group was considerably decrease than that of sodium hydrosulfide solely group at every time level (P<0.05). There have been statistically important variations among the many time factors inside glibenclamide solely group in survival fee of cells (F=11.81, P<0.01).

Culture of 5 min was chosen as the following intervention time of glibenclamide. After 35 min of tradition following the burn serum intervention, in contrast with (1.62±0.08) nmol·min(-1)·mg(-1) and 0.68±0.06 in burn serum management group, the CCO exercise of cells and the protein expression stage of ATP-sensitive potassium channel have been considerably elevated in sodium hydrosulfide solely.

group ((1.99±0.09) nmol·min(-1)·mg(-1) and 0.93±0.01, P<0.01) and considerably decreased in glibenclamide solely group ((1.44±0.09) nmol·min(-1)·mg(-1) and 0.60±0.01, P<0.01); the CCO exercise of cells and the protein expression stage of ATP-sensitive potassium channel in sodium hydrosulfide+glibenclamide group ((1.79±0.06) nmol·min(-1)·mg(-1) and 0.74±0.07) was considerably decrease than these of sodium hydrosulfide solely group (P<0.05 or P<0.01).

Sodium hydrosulfide can enhance the survival fee of rat epidermal cells after the intervention of burn serum, which is expounded to the alleviation of epidermal cell mitochondrial injury and induced by ATP-sensitive potassium ion channel.

[Effects and mechanism of sodium hydrosulfide on rat epidermal cells intervened by burned rat serum]

Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP exercise and Runx2 protein expression

As a flavonoid, rutin has been discovered to have a variety of organic features, comparable to resisting irritation and oxidation, and stopping cerebral hemorrhage and hypertension. It has been discovered to play an necessary function in osteoporosis and different orthopedic illnesses lately. MC3T3-E1 cells have been randomly divided right into a management group, a rutin-1 group (0.01 mmol/L), a rutin-2 group (0.05 mmol/L) and a rutin-Three group (0.1 mmol/L). Osteogenic differentiation of cells was induced by osteogenic induction fluid. The management group was handled with the utmost dose of drug solvent. 2~Three days later, the solvent was changed with contemporary osteogenic induction fluid containing rutin.

After a sure interval of routine tradition, the cells have been collected for subsequent experiments. The expression of Runx2 gene in cells in all teams was detected by Real-time PCR; the expression of Runx2 protein was detected by Western blot and immunocytochemistry; the exercise of ALP was detected by reagent package technique; osteogenic differentiation was analyzed by alizarin purple staining. The outcomes of Real-time PCR confirmed that, in contrast with the management group, the therapy of cells with rutin can considerably enhance the expression of Runx2 gene (p<0.05); the upper the focus, the upper the expression of Runx2 gene, and important variations have been discovered amongst teams wherein totally different concentrations have been used (p<0.05); the outcomes of Western blot and IHC confirmed that the expression development of Runx2 protein in every group was in step with PCR outcomes.

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E05S0273-96 1 plate of 96 wells
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E01A42617 96T
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Description: ELISA

Guinea pig Serum Albumin ELISA kit
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Albumin ELISA kit
E05S0014-48 1 plate of 48 wells
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Albumin ELISA kit
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit
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Guinea pig Serum amyloid ELISA kit
E05S0228-48 1 plate of 48 wells
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit
E05S0228-96 1 plate of 96 wells
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Albumin antibody (HRP)
60R-1144 100 ug
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Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (HRP)

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Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (FITC)

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Description: ELISA

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Description: ELISA

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Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig serum albumin antibody ELISA kit
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E01A42879 96T
EUR 700
Description: ELISA

Guinea pig Serum response factor(SRF) ELISA kit
E05S0401-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E05S0401-48 1 plate of 48 wells
EUR 624
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E05S0401-96 1 plate of 96 wells
EUR 822
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Guinea Pig Serum Amyloid P Component ELISA kit
E01A42735 96T
EUR 700
Description: ELISA

Guinea pig Serum Amyloid P Component ELISA kit
E05S0234-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Amyloid P Component ELISA kit
E05S0234-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Amyloid P Component ELISA kit
E05S0234-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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Guinea Pig Glycosylated serum protein ELISA kit
E01A39754 96T
EUR 700
Description: ELISA

Guinea pig Glycosylated serum protein ELISA kit
E05G0373-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Glycosylated serum protein ELISA kit
E05G0373-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

In drug therapy teams, the exercise of ALP was considerably larger than that within the management group (p<0.05); there have been important variations amongst teams wherein totally different concentrations have been used (p<0.05).

The outcomes of alizarin purple staining confirmed that calcified nodules have been shaped in all teams and that the world of calcified nodules shaped in teams handled with rutin was higher than that within the management group; the higher the focus, the bigger the world. Rutin can promote osteoblastic differentiation; and the higher the focus, the more practical it’s.

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Posted by Sebastian Hernandez on in Antibodies, Assay Kits, Biology Cells, Biosafety Studies of a Clinically Applicable Lentiviral Vector for the Gene Therapy of Artemis-SCID., Clia Kits, Culture Cells, DNA, DNA Templates, DNA Testing, Effect of Medication Co-payment Vouchers on P2Y12 Inhibitor Use and Major Adverse Cardiovascular Events Among Patients With Myocardial Infarction: The ARTEMIS Randomized Clinical Trial., Elisa Kits, Equipments, Exosomes, Gels, Isotypes, My Blog, NATtrol, Panel, Particles, Pcr Kits, Peptides, Reagents, Ria Kits, RNA, Vector & Virus
Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Class II main histocompatibility advanced peptide (MHC-IIp) multimers are exactly engineered reagents used to detect T cells particular for antigens from pathogens, tumors, and self-proteins. While the associated Class I MHC/peptide (MHC-Ip) multimers are often produced from subunits expressed in E. coli, most Class II MHC alleles can’t be produced in micro organism, and this has contributed to the notion that MHC-IIp reagents are tougher to supply.

Herein, we current a sturdy constitutive expression system for soluble biotinylated MHC-IIp proteins that makes use of steady lentiviral vector-transduced derivatives of HEK-293T cells. The expression design contains allele-specific peptide ligands tethered to the amino-terminus of the MHC-II β chain by way of a protease-cleavable linker. Following cleavage of the linker, HLA-DM is used to catalyze environment friendly peptide alternate, enabling high-throughput manufacturing of many distinct MHC-IIp complexes from a single manufacturing cell line.

Peptide alternate is monitored utilizing both of two label-free strategies, native isoelectric focusing gel electrophoresis or matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry of eluted peptides.

Together, these strategies produce MHC-IIp complexes which might be extremely homogeneous and that type the premise for excellent MHC-IIp multimer reagents. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Lentivirus manufacturing and expression line creation Support Protocol 1: Six-well assay for estimation of manufacturing cell line yield Support Protocol 2: Universal ELISA for quantifying proteins with fused leucine zippers and His-tags Basic Protocol 2: Cultures for manufacturing of Class II MHC proteins Basic

Protocol 3: Purification of Class II MHC proteins by anti-leucine zipper affinity chromatography Alternate Protocol 1: IMAC purification of His-tagged Class II MHC Support Protocol 3: Protein focus measurements and changes Support Protocol 4: Polishing purification by anion-exchange chromatography

Support Protocol 5: Estimating biotinylation share by streptavidin precipitation Basic Protocol 4: Peptide alternate Basic Protocol 5: Analysis of peptide alternate by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry Alternate Protocol 2: Native isoelectric focusing to validate MHC-II peptide loading Basic Protocol 6: Multimerization Basic Protocol 7: Staining cells with Class II MHC tetramers.

Autonomous spheroid formation by tradition plate compartmentation

Scaffold-free 3D cell cultures (e.g. pellet cultures) are broadly used in medical science, together with cartilage regeneration. Their drawbacks are excessive time/reagent consumption and lack of early readout parameters. While optimisation was achieved by automation or simplified spheroid era, most tradition methods stay costly or require tedious procedures. The purpose of this research was to ascertain a system for useful resource environment friendly spheroid era.

This was achieved by compartmentation of cell tradition surfaces utilising laser engraving (grid plates). This compartmentation triggered autonomous spheroid formation by way of rolling-up of the cell monolayer in human adipose-derived stem cells (ASC/TERT1) and human articular chondrocytes (hAC)-ASC/TERT1 co-cultures, when cultivated on grid plates below chondrogenic circumstances. Plates with Three mm grid dimension yielded steady diameters (about 300 μm). ASC/TERT1 spheroids totally shaped inside

Three weeks whereas co-cultures took 1-2 weeks, forming considerably quicker with rising hAC ratio (p<0.05 and 0.01 for 1:1 and 1:Four ASC/TERT1:hAC ratio respectively). Co-cultures confirmed barely decrease spheroid diameter, attributable to earlier spheroid formation and incomplete monolayer formation. However, this was related to extra common matrix distribution in the co-tradition.

Both confirmed differentiation capability comparable to plain pellet tradition in (immune-)histochemistry and RT-qPCR. To assess usability for cartilage restore, spheroids have been embedded right into a hydrogel (fibrin), yielding cellular outgrowth and matrix deposition, which was particularly pronounced in co-cultures. The herein offered novel cell tradition system is just not solely a promising instrument for autonomous spheroid era with the potential of experimental and scientific software in tissue engineering but in addition for high-throughput evaluation for each pharmaceutical and therapeutic makes use of.

Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Development of a novel β-1,6-glucan-specific detection system utilizing functionally-modified recombinant endo-β-1,6-glucanase

β-1,3D-Glucan is a ubiquitous glucose polymer produced by vegetation, micro organism, and most fungi. It has been used as a diagnostic instrument in sufferers with invasive mycoses by way of a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no methodology is presently obtainable for measuring β-1,6-glucan, one other main β-glucan construction of fungal polysaccharides.

Herein, we describe the event of a cheap and highly-sensitive and particular assay for β-1,6-glucan utilizing a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase exercise. The purified β-1,6-glucanase by-product certain to the β-1,6-glucan pustulan with a OkayD of 16.Four nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its capacity to detect cell wall β-1,6-glucan from each yeast and hyphal types of the opportunistic fungal pathogen Candida albicans, with none detectable binding to glucan missing the lengthy β-1,6-glucan department.

RABBIT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00017 20 ml Ask for price

RABBIT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00018 100 ml Ask for price

GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00004 5 ml Ask for price

OOSA10412-5ML - SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10412-5ML 5ml
EUR 69

OOSA10414-10ML - SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10414-10ML 10ml
EUR 149

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10429-10ML 10ml
EUR 279

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10433-100ML 100ml
EUR 1799

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP 30 rxn
EUR 1201.2

Goat Serum (10%) in PBST with Azide
30611260-1 100 ml
EUR 33.41

Goat Serum (10%) in PBST with Azide
30611260-2 250 ml
EUR 59.36

Horse Serum (10%) in PBST with Azide
30611278-1 100 ml
EUR 31.76

Horse Serum (10%) in PBST with Azide
30611278-2 250 ml
EUR 59.79

Goat Serum (10%) in TBST Buffer with Azide
30611263-1 100 mL
EUR 33.41

Goat Serum (10%) in TBST Buffer with Azide
30611263-2 250 ml
EUR 57.19

Horse Serum (10%) in PBS with Azide, pH 7.4
40120170-1 100 ml
EUR 31.76

Horse Serum (10%) in PBS with Azide, pH 7.4
40120170-2 250 ml
EUR 54.54

Goat Serum (10%) in TBST Wash Buffer with Azide
30611266-1 100 ml
EUR 33.41

Goat Serum (10%) in TBST Wash Buffer with Azide
30611266-2 250 ml
EUR 57.11

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile
30611251-1 100 ml
EUR 32.94

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile
30611251-2 250 ml
EUR 59.44

Horse Serum (10%) in TBST with Sodium Azide, Sterile
30611281-1 100 ml
EUR 31.76

Horse Serum (10%) in TBST with Sodium Azide, Sterile
30611281-2 250 ml
EUR 54.54

VEX Exosome Isolation Reagent (from serum)
R602 10 ml
EUR 769.2

Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611257-1 100 ml
EUR 33.41

Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611257-2 250 ml
EUR 57.11

Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611275-1 50 ml
EUR 36.77

Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611275-2 100 ml
EUR 54.54

Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611254-1 100 ml
EUR 33.41

Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611254-2 250 ml
EUR 57.11

Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611272-1 50 ml
EUR 31.67

Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611272-2 100 ml
EUR 54.64

Bovine Serum Albumin Fatty Acid Free Reagent Grade
GWB-F06E29 1 kg Ask for price

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002M 25ml/BT
EUR 800

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002S 1ml*5/set
EUR 200

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-5 each
EUR 144

Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile
30611283-1 100 ml
EUR 31.76

Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile
30611283-2 250 ml
EUR 54.54

MOUSE SERUM:Azide Free Serum Products
GWB-Q00010 5 ml Ask for price

MOUSE SERUM:Azide Free Serum Products
GWB-Q00011 10 ml Ask for price

MOUSE SERUM:Azide Free Serum Products
GWB-Q00012 50 ml Ask for price

MOUSE SERUM:Azide Free Serum Products
GWB-Q00013 100 ml Ask for price

MOUSE SERUM:Azide Free Serum Products
GWB-Q00014 500 ml Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T 50 T Ask for price

Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!
P5W13 10 rxn
EUR 99

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade
OPEF01351-100G 100g
EUR 1302

Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade
GWB-DCC190 1 kg Ask for price

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)
WA-013 each
EUR 135

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: SPM376
APR07282G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: ALB/398
APR07283G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

MOUSE SERUM Serum Products
GWB-Q00006 100 ml Ask for price

EZ-DNA Reagents
BS8202 100preps
EUR 105.67

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

DOG SERUM:Azide free Serum Products
GWB-Q00003 100 ml Ask for price

RAT SERUM:Azide Free Serum Products
GWB-Q00024 10 ml Ask for price

RAT SERUM:Azide Free Serum Products
GWB-Q00025 50 ml Ask for price

RAT SERUM:Azide Free Serum Products
GWB-Q00026 100 ml Ask for price

RABBIT SERUM:Azide Free Serum Products
GWB-Q00019 20 ml Ask for price

RABBIT SERUM:Azide Free Serum Products
GWB-Q00020 100 ml Ask for price

Ecl Detection Reagents
RPN3004 EACH
EUR 552.9

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-1 1 mg
EUR 169.2

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-5 5 mg
EUR 343.2

Mouse Serum
abx098261-100L 100 L
EUR 155407.2

Mouse Serum
20-abx098261
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Mouse Serum
abx098261-5L 5 L
EUR 10599.6

Mouse Serum
abx098262-1ml 1 ml
EUR 2064

Mouse Serum
abx098261-1ml 1 ml Ask for price

Mouse Serum
abx098261-200l 200 µl Ask for price

Mouse serum
IHR-8141 10 ml
EUR 47.75

Mouse Serum
M2021-050 500ml
EUR 970

Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile
30611269-1 50 mL
EUR 36.77

Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile
30611269-2 100 ml
EUR 57.64

Mouse Serum - 100ml
MO-340/100 100ml
EUR 183.32

Mouse Serum - 500ml
MO-340/500 500ml
EUR 733.32

GUINEA PIG SERUM:Azide Free Serum Products
GWB-Q00005 5 ml Ask for price

Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: SPM376
APR07284G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E

OOSA10411-5ML - SERUM:Azide Free Serum & Cells
OOSA10411-5ML 5ml
EUR 99

Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: ALB/398
AMM04382G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

OOSA10417-50ML - SERUM:Azide Free Serum & Cells
OOSA10417-50ML 50ml
EUR 469

OOSA10426-20ML - SERUM:Azide Free Serum & Cells
OOSA10426-20ML 20ml
EUR 99

OOSA10430-10ML - SERUM:Azide Free Serum & Cells
OOSA10430-10ML 10ml
EUR 379

OOSA10419-100ML - SERUM:Azide Free Serum & Cells
OOSA10419-100ML 100ml
EUR 749

OOSA11794-100ML - SERUM:Azide free Serum & Cells
OOSA11794-100ML 100ml
EUR 1489

Ecl Detection Reagents - EACH
RPN2105 EACH
EUR 815.4

Mouse Serum Proteins goat polyclonal antibody, Serum
AP31483SU-N 1 ml Ask for price

Mouse Serum Proteins rabbit polyclonal antibody, Serum
AP31472SU-N 1 ml Ask for price

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Mouse Serum Amyloid P Component (SAP) Reference serum
SAP12-RS 100 ul
EUR 388.8

Mouse Serum - 10ml
MO-340/10 10ml
EUR 28.11

Mouse Serum - 20ml
MO-340/20 20ml
EUR 55

Mouse Serum - 50ml
MO-340/50 50ml
EUR 97.79

Human IgG (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-1 1 mg
EUR 196.8

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Normal Mouse Serum
88-NM35 50 ml
EUR 459
Description: Normal Mouse Serum

Normal Mouse Serum
88R-1002 5 ml
EUR 118
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Mouse Serum
BMS0070-10mL 10 mL
EUR 89
Description: High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays.

Normal Mouse Serum
BMS0070-10mL10 10 mL×10
EUR 529
Description: High quality Normal mouse serum processed from non-immunized normal adult mouses is used as a blocking agent or as a negative control for immunoassays.

Normal Mouse Serum
NMS 1 ml/vial
EUR 91

Normal Mouse Serum
BMS0070-each each Ask for price

Mouse Serum Albumin
30R-3304 10 mg
EUR 153
Description: Purified native Mouse Serum Albumin

Mouse Serum Albumin
CSB-NP000801m 10mg Ask for price

REAGENTS HIGH RANGE AMMONIA - PK25
HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH
HI9373301 EACH
EUR 130.95

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

PeliKine tool set, additional reagents
M1980 1 unit
EUR 169.8

Mouse Complement Serum
32R-CM001 5 ml
EUR 526
Description: Frozen high activity mouse complement serum

Alkphos Direct Labelling Reagents - EACH
RPN3680 EACH
EUR 915.3

VACUETTE Bleeding with Gel Activator Serum 8ml Greiner VACUETTE Bleeding with Gel Activator Serum 8ml Greiner - PK50
455071A PK50
EUR 62.1

Mouse Balb C Serum
IMSBCSER1000ML each
EUR 2265
Description: Mouse Balb C Serum

Mouse Balb C Serum
IMSBCSER100ML each
EUR 368
Description: Mouse Balb C Serum

Mouse Balb C Serum
IMSBCSER10ML each
EUR 70
Description: Mouse Balb C Serum

Mouse Balb C Serum
IMSBCSER25ML each
EUR 155
Description: Mouse Balb C Serum

Mouse Balb C Serum
IMSBCSER500ML each
EUR 1199
Description: Mouse Balb C Serum

Mouse Balb C Serum
IMSBCSER50ML each
EUR 187
Description: Mouse Balb C Serum

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-BTN 100 ug
EUR 343.2

Mouse Serum albumin (Alb)
1-CSB-YP001561MO
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
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Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast

Mouse Serum albumin (Alb)
1-CSB-BP001561MO
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus

Free Chlorine Reagents 0-5mg/L - EACH
WAT1028 EACH
EUR 49.95

Non-sterile mouse serum
MS05-0050 50 ml
EUR 156.25

Non-sterile mouse serum
MS05-0100 100 ml
EUR 188.75

Non-sterile mouse serum
MS05-0500 500 ml
EUR 531.25

*Mouse IgG Depleted Serum
IMSIGGDS100ML each
EUR 3730
Description: *Mouse IgG Depleted Serum

*Mouse IgG Depleted Serum
IMSIGGDS10ML each
EUR 466
Description: *Mouse IgG Depleted Serum

Mouse Serum Albumin protein
30-1179 10 mg
EUR 234
Description: Purified native Mouse Albumin protein

Mouse Serum Albumin Protein
20-abx600021
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  • 100 mg
  • 10 mg
  • 1 g
  • 1 mg

Normal Mouse Serum Filtered
GWB-E7645B 10 ml Ask for price

Mouse Serum Albumin Protein
abx600021-100g 100 µg
EUR 237.5

Mouse Serum Albumin Protein
abx600021-200g 200 µg
EUR 837.5

Mouse Serum Albumin Protein
abx600021-50g 50 µg
EUR 175

Mouse Serum Albumin antibody
20R-AR005 20 mg
EUR 387
Description: Rabbit polyclonal Mouse Serum Albumin antibody

Mouse Serum albumin antibody
20R-AR041 2 ml
EUR 343
Description: Rabbit polyclonal Mouse Serum albumin antibody

Mouse Serum Albumin antibody
70R-AG001 1 mg
EUR 209
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody

Mouse Serum Lipid-Stripped
GWB-D35A77 1 l Ask for price

WASH SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-784-CM 1 L /pk
EUR 85.2
Description: Specialty Media; Islet products

OOSA10418-100ML - SERUM Serum & Cells
OOSA10418-100ML 100ml
EUR 709

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050 450ml
EUR 225.6

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-100 950ml
EUR 130

Mouse Normal Serum, Antibody
GWB-9A9CFB 0.05 ml Ask for price

Mouse Serum Albumin, Lyophilized
22070104-1 100 mg
EUR 261.45

Mouse Serum Albumin, Lyophilized
22070104-2 500 mg
EUR 822.66

Mouse Serum Albumin, Lyophilized
22070104-3 1000 mg
EUR 1280.2

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-783-CM 1 L /pk
EUR 75.6
Description: Specialty Media; Islet products

Mouse Serum Iron ELISA kit
E03S0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Iron ELISA kit
E03S0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Iron ELISA kit
E03S0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Liposomal and Non-Liposomal Transfection Reagents
abx298032-100g 100 µg Ask for price

Liposomal and Non-Liposomal Transfection Reagents
abx298032-20g 20 µg
EUR 875

Liposomal and Non-Liposomal Transfection Reagents
abx298032-50g 50 µg Ask for price

Mouse Serum Amyloid P ELISA
KT-347 1 kit
EUR 551

Mouse Serum Iron (SIRON) ELISA
KT-58966 96 tests
EUR 981

Mouse control serum, CD-1
NMOS-CD1-1 1 ml
EUR 123.6

Mouse control serum, CD-1
NMOS-CD1-5 5 ml
EUR 196.8

Serum Amyloid A Mouse mAb
E2222727 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Mouse Serum Albumin ELISA kit
E01A25178 96T
EUR 700
Description: ELISA

Mouse Serum Albumin ELISA kit
E03S0014-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Albumin ELISA kit
E03S0014-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum Albumin ELISA kit
E03S0014-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum amyloid ELISA kit
E03S0228-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum amyloid ELISA kit
E03S0228-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Serum amyloid ELISA kit
E03S0228-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Fetal Bovine Serum（Without Exosome ）
FBSNE-01061 20/50mL Ask for price

Mouse control serum, Balb/c
NMOS-5BS 5 ml
EUR 196.8

We developed a sandwich ELISA-like assay with a low restrict of quantification for pustulan (1.5 pg/ml), and we efficiently employed this assay in the quantification of furthercellular β-1,6-glucan launched by >250 patient-derived strains of completely different Candida species (together with Candida auris) in tradition supernatant in vitro. We additionally used this assay to measure β-1,6-glucan in vivo in the serum and in a number of organs in a mouse mannequin of systemic candidiasis. Our work describes a dependable methodology for β-1,6-glucan detection, which can show helpful for the prognosis of invasive fungal infections.